ArticleThe Journal of cell biology1983
Elongation of the fertilization tubule in Chlamydomonas: new observations on the core microfilaments and the effect of transient intracellular signals on their structural integrity.
Article in The Journal of cell biology, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 91 citations in OpenAlex.
- HAP2-Mediated Gamete Fusion: Lessons From the World of Unicellular Eukaryotes.Frontiers in cell and developmental biology · 2021Review
- Diversity from similarity: cellular strategies for assigning particular identities to actin filaments and networks.Open biology · 2020Article
- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
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- Sex-specific posttranslational regulation of the gamete fusogen GCS1 in the isogamous volvocine alga Gonium pectorale.Eukaryotic cell · 2014Article
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- Gsp1 triggers the sexual developmental program including inheritance of chloroplast DNA and mitochondrial DNA in Chlamydomonas reinhardtii.The Plant cell · 2012Article
- Uniparental inheritance of cpDNA and the genetic control of sexual differentiation in Chlamydomonas reinhardtii.Journal of plant research · 2010Review
- The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes.Genes & development · 2008Article
- The transcriptional program of synchronous gametogenesis in Chlamydomonas reinhardtii.Current genetics · 2004Article
- Expression of conventional and unconventional actins in Chlamydomonas reinhardtii upon deflagellation and sexual adhesion.Eukaryotic cell · 2003Article
- The Chlamydomonas Fus1 protein is present on the mating type plus fusion organelle and required for a critical membrane adhesion event during fusion with minus gametes.Molecular biology of the cell · 2003Article
- The Chlamydomonas mating type plus fertilization tubule, a prototypic cell fusion organelle: isolation, characterization, and in vitro adhesion to mating type minus gametes.The Journal of cell biology · 1997Article
- Chlamydomonas inner-arm dynein mutant, ida5, has a mutation in an actin-encoding gene.The Journal of cell biology · 1997Article
- Cell adhesion-dependent inactivation of a soluble protein kinase during fertilization in Chlamydomonas.Molecular biology of the cell · 1996Article
- Inner dynein arms but not outer dynein arms require the activity of kinesin homologue protein KHP1(FLA10) to reach the distal part of flagella in Chlamydomonas.The Journal of cell biology · 1996Article
- Signal transduction in the sexual life of Chlamydomonas.Plant molecular biology · 1994Review
- The role of calcium in the Chlamydomonas reinhardtii mating reaction.The Journal of cell biology · 1993Article
- Flagellar microtubule dynamics in Chlamydomonas: cytochalasin D induces periods of microtubule shortening and elongation; and colchicine induces disassembly of the distal, but not proximal, half of the flagellum.The Journal of cell biology · 1992Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Experimental manipulations of gametes of Chlamydomonas reinhardi and ultrastructural observation were used to examine the composition of the microfilaments in the fertilization tubule, their probable mode of formation, and their interaction with intracellular signals. Decoration with myosin subfragment-1 was used to demonstrate that the microfilaments in the fertilization tubule were actin filaments having uniform polarity: Myosin subfragment-1 arrowheads pointed away from the membrane at the tip of the process. Filaments were attached to the cone-shaped "doublet zone" at the base of the process by their pointed ends. Discrete attachment sites for filaments on the surface of the doublet zone were seen in stereo view. To test whether actin polymerization might accompany elongation of the fertilization tubule, mating gametes were exposed to cytochalasin D in an attempt to block actin polymerization. Treatment of mating type "plus" gametes with cytochalasin D prior to and during mating inhibited the appearance of actin filaments in fertilization tubules, suppressed fertilization tubule outgrowth, and lowered mating efficiency from 90 to 15%. The role of signals generated by flagellar adhesion in maintaining the structural integrity of the microfilament-doublet zone complex was examined by correlating flagellar disadhesion with the kinetics of breakdown of the complex. In zygotes, where flagellar disadhesion occurred after cell fusion, the complex disassembled within 3 h after mating. In gametes that had been agglutinated by isolated mating type "minus" flagella, microfilaments and fertilization tubules progressively disassembled over a 3-h time course following flagellar disadhesion. Disassembly of microfilaments was inhibited by maintaining flagellar agglutination, suggesting that signals generated by flagellar adhesion were necessary to maintain microfilaments intact.
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