ArticleThe Journal of cell biology1984
A membrane cytoskeleton from Dictyostelium discoideum. III. Plasma membrane fragments bind predominantly to the sides of actin filaments.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Ponticulin is an atypical membrane protein.The Journal of cell biology · 1994Article
- Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium.The Journal of cell biology · 1994Article
- Binding of actin to liver cell membranes: the state of membrane-bound actin.The Journal of cell biology · 1991Article
- Dictyostelium discoideum plasma membranes contain an actin-nucleating activity that requires ponticulin, an integral membrane glycoprotein.The Journal of cell biology · 1990Article
- How actin binds and assembles onto plasma membranes from Dictyostelium discoideum.The Journal of cell biology · 1988Article
- F-actin binds to the cytoplasmic surface of ponticulin, a 17-kD integral glycoprotein from Dictyostelium discoideum plasma membranes.The Journal of cell biology · 1987Article
- Calcium-sensitive, lipid-binding cytoskeletal proteins of the human placental microvillar region.The Journal of cell biology · 1987Article
- Cell surface proteoglycan associates with the cytoskeleton at the basolateral cell surface of mouse mammary epithelial cells.The Journal of cell biology · 1986Article
- Binding and assembly of actin filaments by plasma membranes from Dictyostelium discoideum.The Journal of cell biology · 1986Article
- An integral glycoprotein associated with the membrane attachment sites of actin microfilaments.The Journal of cell biology · 1985Article
- Ligand-induced changes in the location of actin, myosin, 95K (alpha-actinin), and 120K protein in amebae of Dictyostelium discoideum.The Journal of cell biology · 1985Article
- Decoration with myosin subfragment-1 disrupts contacts between microfilaments and the cell membrane in isolated Dictyostelium cortices.The Journal of cell biology · 1984Article
- A membrane cytoskeleton from Dictyostelium discoideum. II. Integral proteins mediate the binding of plasma membranes to F-actin affinity beads.The Journal of cell biology · 1984Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The binding between sonicated Dictyostelium discoideum plasma membrane fragments and F-actin on Sephacryl S-1000 beads was found to be competitively inhibited by myosin subfragment-1. This inhibition is MgATP-sensitive, exhibits a Ki of approximately 5 X 10(-8) M, and is reciprocal, since membranes inhibit the binding of 125I-heavy meromyosin to F-actin on beads. These experiments demonstrate that membrane binding and S-1 binding to F-actin on beads are mutually exclusive and, therefore, that the membrane fragments bind predominantly to the sides, rather than to the ends, of the actin filaments. This conclusion is supported by electron micrographs that show many lateral associations between membrane fragments and bead-associated actin filaments. Such lateral associations could play an important role in the organization and lateral movement of membrane proteins by the cytomusculature.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.