ArticleThe Journal of cell biology1983
Evidence for mediated protein uptake by amphibian oocyte nuclei.
Article in The Journal of cell biology, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 62 citations in OpenAlex.
- Toxoplasma Gondii Importin α Shows Weak Auto-Inhibition.The protein journal · 2023Article
- Shiga toxin is transported into the nucleoli of intestinal epithelial cells via a carrier-dependent process.Toxins · 2010Article
- Nuclear import of Avian Sarcoma Virus integrase is facilitated by host cell factors.Retrovirology · 2008Article
- Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein.The Journal of cell biology · 1992Article
- Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.The Journal of cell biology · 1990Article
- Characterization of nuclear localizing sequences derived from yeast ribosomal protein L29.The EMBO journal · 1990Article
- The effects of variations in the number and sequence of targeting signals on nuclear uptake.The Journal of cell biology · 1988Article
- A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.The Journal of cell biology · 1988Article
- Identification of domains involved in nuclear uptake and histone binding of protein N1 of Xenopus laevis.The EMBO journal · 1988Article
- Translocation of RNA-coated gold particles through the nuclear pores of oocytes.The Journal of cell biology · 1988Article
- The nuclear migration signal of Xenopus laevis nucleoplasmin.The EMBO journal · 1987Article
- Permeability measurements with closed vesicles from rat liver nuclear envelopes.Proceedings of the National Academy of Sciences of the United States of America · 1987Article
- Monoclonal antibodies identify a group of nuclear pore complex glycoproteins.The Journal of cell biology · 1987Article
- Nuclear-envelope vesicles as a model system to study nucleocytoplasmic transport. Specific uptake of nuclear proteins.The Biochemical journal · 1987Article
- Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.The EMBO journal · 1986Article
- In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins.The Journal of cell biology · 1986Article
- Determination of the intracellular state of soluble macromolecules by gel filtration in vivo in the cytoplasm of amphibian oocytes.The Journal of cell biology · 1986Article
- Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation.The EMBO journal · 1986Article
- Movement of a karyophilic protein through the nuclear pores of oocytes.The Journal of cell biology · 1984Article
- Antibodies to a nucleolar protein are localized in the nucleolus after red blood cell-mediated microinjection.The Journal of cell biology · 1983Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this investigation was to determine whether there is mediated transport of endogenous proteins across the nuclear envelope. For this purpose, we studied the nuclear uptake of a 148,000-dalton Rana oocyte polypeptide (RN1) and compared its actual uptake rate with the rate that would be expected if RN1 crossed the envelope by simple diffusion through the nuclear pores. Nuclear uptake was studied in two ways: first, oocytes were incubated in L-[3H]leucine for 1 h and, at various intervals after labeling, the amount of 3H-RN1 present in the nucleoplasm was determined. Second, L-[3H]leucine-labeled nuclear extracts, containing RN1, were microinjected into the cytoplasm of nonlabeled cells, and the proportion of 3H-RN1 that subsequently entered the nucleus was measured. It was found that RN1 can readily penetrate the nuclear envelope; for example, after 6 h, approximately 36% of the newly synthesized RN1 and 17% of the injected RN1 had entered the nucleus. The diffusion rate through pores having a radius of 45 A was calculated for several possible molecular configurations of RN1. Using axial ratios of 34, 7.5, 2, and 1, the estimated times required to reach 63% of diffusion equilibrium are 757, 468, 6,940 h, and infinity, respectively. Even assuming an axial ratio of 7.5 (the most diffusive configuration) and an equilibrium distribution of 45, simple diffusion through the pores could account for only approximately 1/20 the observed nuclear uptake of RN1. This and other comparisons indicate that some form of mediated transport is involved in the nucleocytoplasmic exchange of this polypeptide.
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