ArticleThe Journal of cell biology1983
Mechanism of brush border contractility studied by the quick-freeze, deep-etch method.
Article in The Journal of cell biology, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed, 147 citations in OpenAlex.
- Unique and redundant functions of cytoplasmic actins and nonmuscle myosin II isoforms at epithelial junctions.Annals of the New York Academy of Sciences · 2022Review
- Microscopic Visualization of Cell-Cell Adhesion Complexes at Micro and Nanoscale.Frontiers in cell and developmental biology · 2022Review
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- Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules.Biophysical journal · 2019Article
- Multiscale dynamics of tight junction remodeling.Journal of cell science · 2019Review
- Hypoxanthine is a checkpoint stress metabolite in colonic epithelial energy modulation and barrier function.The Journal of biological chemistry · 2018Article
- Synaptopodin couples epithelial contractility to α-actinin-4-dependent junction maturation.The Journal of cell biology · 2015Article
- A complex of ZO-1 and the BAR-domain protein TOCA-1 regulates actin assembly at the tight junction.Molecular biology of the cell · 2015Article
- Control of creatine metabolism by HIF is an endogenous mechanism of barrier regulation in colitis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- αE-catenin actin-binding domain alters actin filament conformation and regulates binding of nucleation and disassembly factors.Molecular biology of the cell · 2013Article
- A WAVE2-Arp2/3 actin nucleator apparatus supports junctional tension at the epithelial zonula adherens.Molecular biology of the cell · 2012Article
- A bigger picture: classical cadherins and the dynamic actin cytoskeleton.Nature reviews. Molecular cell biology · 2012Review
- Nonredundant roles of cytoplasmic β- and γ-actin isoforms in regulation of epithelial apical junctions.Molecular biology of the cell · 2012Article
- Myosin Ia is required for CFTR brush border membrane trafficking and ion transport in the mouse small intestine.Traffic (Copenhagen, Denmark) · 2012Article
- Myosin-X functions in polarized epithelial cells.Molecular biology of the cell · 2012Article
- The receptor tyrosine phosphatase Lar regulates adhesion between Drosophila male germline stem cells and the niche.Development (Cambridge, England) · 2012Article
- Regulation of epithelial permeability by the actin cytoskeleton.Cytoskeleton (Hoboken, N.J.) · 2011Review
- N-WASP regulates the epithelial junctional actin cytoskeleton through a non-canonical post-nucleation pathway.Nature cell biology · 2011Article
- Cytoskeletal regulation of epithelial barrier function during inflammation.The American journal of pathology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed terminal web contraction in sheets of glycerinated chicken small intestine epithelium and in isolated intestinal brush borders using a quick-freeze, deep-etch, rotary shadow replication technique. In the presence of Mg-ATP at 37 degrees C, the terminal web region of each cell in the glycerinated sheet and of each isolated brush border became severely constricted at the level of its zonula adherens (ZA). Consequently, the individual brush borders rounded up, splaying out their microvilli in fanlike patterns. The most prominent ultrastructural changes that occurred during terminal web contraction were a dramatic decrease in the diameter of the circumferential ring composed of a bundle of 8-9-nm filaments adjacent to the zonula adherens and a decrease in the number of cross-linkers between the microvillus rootlets. Microvilli were not retracted into the terminal web. We have used myosin S1 decoration to demonstrate that most of the circumferential bundle filaments are actin and that the actin filaments are arranged in the bundle with mixed polarity. Some filaments within the bundle did not decorate with myosin S1 and had tiny projections that appeared to be attached to adjacent actin filaments. Because of their morphology and immunofluorescent localization of myosin within this region of the terminal web, we propose that these undecorated filaments are myosin. From these results, we conclude that brush border contraction is caused primarily by an active sliding of actin and myosin filaments within the circumferential bundle of filaments associated with the ZA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.