ArticleProceedings of the National Academy of Sciences of the United States of America1983
12-fold difference between the critical monomer concentrations of the two ends of actin filaments in physiological salt conditions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Actin network evolution as a key driver of eukaryotic diversification.Journal of cell science · 2024Review
- Solutes unmask differences in clustering versus phase separation of FET proteins.Nature communications · 2024Article
- CKAP5 enables formation of persistent actin bundles templated by dynamically instable microtubules.Current biology : CB · 2024Article
- Mechanisms of actin disassembly and turnover.The Journal of cell biology · 2023Review
- The Actin Regulators Involved in the Function and Related Diseases of Lymphocytes.Frontiers in immunology · 2022Review
- Mitochondrial dynamics, positioning and function mediated by cytoskeletal interactions.Cellular and molecular life sciences : CMLS · 2021Review
- Actin at stereocilia tips is regulated by mechanotransduction and ADF/cofilin.Current biology : CB · 2021Article
- Tropomodulin-3 is essential in asymmetric division during mouse oocyte maturation.Scientific reports · 2016Article
- FtsZ filament capping by MciZ, a developmental regulator of bacterial division.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Reconstitution of a prokaryotic minus end-tracking system using TubRC centromeric complexes and tubulin-like protein TubZ filaments.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Actin structure and function.Annual review of biophysics · 2011Review
- Characterization of the biochemical properties and biological function of the formin homology domains of Drosophila DAAM.The Journal of biological chemistry · 2010Article
- Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes.Molecular biology of the cell · 1997Article
- Role of actin polymerization in cell locomotion: molecules and models.American journal of respiratory cell and molecular biology · 1993Review
- Actin-membrane coupling: a role for talin.Journal of muscle research and cell motility · 1992Article
- The control of actin nucleotide exchange by thymosin beta 4 and profilin. A potential regulatory mechanism for actin polymerization in cells.Molecular biology of the cell · 1992Article
- Domain structure in actin-binding proteins: expression and functional characterization of truncated severin.The Journal of cell biology · 1991Article
- Mechanism of the insertion of actin monomers between the barbed ends of actin filaments and barbed end-bound insertin.Journal of muscle research and cell motility · 1991Article
- ADP-ribosylation of actin by clostridial toxins.The Journal of cell biology · 1989Review
- Probing nucleation, cutting and capping of actin filaments.Journal of muscle research and cell motility · 1989Review
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We determined the critical monomer concentrations at which association and dissociation reactions are balanced at the two ends of actin filaments. For measurement of the critical concentration of the pointed end, interference with the high dynamics of the barbed end was excluded by capping the barbed ends with an actin filament capping protein isolated from bovine brain. The critical concentration of the pointed end (1.5 microM) was found to be 12- to 15-fold higher than the critical concentration of the barbed end (0.10-0.12 microM) at a temperature of 37 degrees C and physiological salt concentrations (100 mM KCl/1-2 mM MgCl2/0.3 mM EGTA or 0.2 mM CaCl2, pH 7.5).
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