ArticleThe Journal of cell biology1984
Epithelial structure revealed by chemical dissection and unembedded electron microscopy.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 55 citations in OpenAlex.
- More complete polarization of renal tubular epithelial cells by artificial urine.Cell death discovery · 2018Article
- CXCL11 Expression by Keratinocytes Occurs Transiently Between Reaching Confluence and Cellular Compaction.Advances in wound care · 2016Article
- Nuclear shape changes are induced by knockdown of the SWI/SNF ATPase BRG1 and are independent of cytoskeletal connections.PloS one · 2013Article
- Phosphorylation of podocalyxin (Ser415) Prevents RhoA and ezrin activation and disrupts its interaction with the actin cytoskeleton.The American journal of pathology · 2011Article
- Novel association of APC with intermediate filaments identified using a new versatile APC antibody.BMC cell biology · 2009Article
- Extracellular matrix, nuclear and chromatin structure, and gene expression in normal tissues and malignant tumors: a work in progress.Advances in cancer research · 2007Review
- Altered profiles of nuclear matrix proteins during the differentiation of human gastric mucous adenocarcinoma MGc80-3 cells.World journal of gastroenterology · 2005Article
- Loss of glomerular foot processes is associated with uncoupling of podocalyxin from the actin cytoskeleton.The Journal of clinical investigation · 2001Article
- Structural protein 4.1 in the nucleus of human cells: dynamic rearrangements during cell division.The Journal of cell biology · 1997Article
- Osteocalcin gene promoter-binding factors are tissue-specific nuclear matrix components.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Resinless section electron microscopy of HeLa cell mitotic architecture.Proceedings of the National Academy of Sciences of the United States of America · 1986Article
- Keratin-like proteins that coisolate with intermediate filaments of BHK-21 cells are nuclear lamins.Proceedings of the National Academy of Sciences of the United States of America · 1986Article
- Cocompartmentation of proteins and K+ within the living cell.Proceedings of the National Academy of Sciences of the United States of America · 1986Article
- Cytoskeletal properties and endogenous degradation of glial fibrillary acidic protein and vimentin in cultured human glioma cells.Acta neuropathologica · 1986Article
- Cytomatrix in chromatophores.The Journal of cell biology · 1984Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cytoskeletal structures obtained after extraction of Madin-Darby canine kidney epithelial cell monolayers with Triton X-100 were examined in transmission electron micrographs of cell whole mounts and unembedded thick sections. The cytoskeleton, an ordered structure consisting of a peripheral plasma lamina, a complex network of filaments, and chromatin-containing nuclei, was revealed after extraction of intact cells with a nearly physiological buffer containing Triton X-100. The cytoskeleton was further fractionated by extraction with (NH4)2SO4, which left a structure enriched in intermediate filaments and desmosomes around the nuclei. A further digestion with nuclease and elution with (NH4)2SO4 removed the chromatin. The stable structure that remained after this procedure retained much of the epithelial morphology and contained essentially all of the cytokeratin filaments and desmosomes and the chromatin-depleted nuclear matrices. This structural network may serve as a scaffold for epithelial organization. The cytoskeleton and the underlying nuclear matrix intermediate filament scaffold, when examined in both conventional embedded thin sections and in unembedded whole mounts and thick sections, showed the retention of many of the detailed morphological aspects of the intact cells, which suggests a structural continuum linking the nuclear matrix, the intermediate filament network, and the intercellular desmosomal junctions. Most importantly, the protein composition of each of the four fractions obtained by this sequential procedure was essentially unique. Thus, the proteins constituting the soluble fraction, the cytoskeleton, the chromatin fraction, and the underlying nuclear matrix-intermediate filament scaffold are biochemically distinct.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.