ArticleBiophysical journal1984
Separation of translational and rotational contributions in solution studies using fluorescence photobleaching recovery.
Article in Biophysical journal, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 31 citations in OpenAlex.
- Burst-by-Burst Measurement of Rotational Diffusion at Nanosecond Resolution Reveals Hot-Brownian Motion and Single-Chain Binding.ACS nano · 2023Article
- Continuous Fluorescence Depletion Anisotropy Measurement of Protein Rotation.Journal of fluorescence · 2018Article
- A new method for diffusion measurement in polymeric films based on a stacked sheet concept.Pharmaceutical research · 1996Article
- Myosin head rotation in muscle fibers measured using polarized fluorescence photobleaching recovery.Journal of fluorescence · 1995Article
- Saturation effects in polarized fluorescence photobleaching recovery and steady state fluorescence polarization.Biophysical journal · 1994Article
- Protein rotational dynamics investigated with a dual EPR/optical molecular probe. Spin-labeled eosin.Biophysical journal · 1993Article
- Slow rotational mobilities of antibodies and lipids associated with substrate-supported phospholipid monolayers as measured by polarized fluorescence photobleaching recovery.Biophysical journal · 1990Article
- Separation of the rotational contribution in fluorescence correlation experiments.Biophysical journal · 1989Article
- Polarized fluorescence photobleaching recovery for measuring rotational diffusion in solutions and membranes.Biophysical journal · 1988Article
- A fluorescence photobleaching study of the microsecond reorientational motions of DNA.Biophysical journal · 1988Article
- Fluorescence recovery spectroscopy as a probe of slow rotational motions.Biophysical journal · 1984Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Although fluorescence photobleaching recovery (FPR) experiments are usually interpreted in terms of the translational motions of a fluorescently labeled species, rotational motions can also modulate recovery through the cosine-squared laws for dipolar absorption and emission processes. In a complex interacting system, translational and rotational contributions may both be simultaneously present. We show how these contributions can be separated in solution studies using an FPR setup in which (a) the linear polarization of the low-intensity observation beam and the high-intensity photobleaching pulse can be varied independently, and (b) all emitted fluorescent photons are counted equally. The fluorescence recovery signal obtained with the observation beam polarized at the magic angle, 54.7 degrees, from the bleach polarization direction is independent of label orientation, whereas the anisotropy function formed from a combination of parallel and perpendicular polarizations isolates the orientational recovery. The anisotropy function is identical to that in fluorescence correlation spectroscopy and, for rigid-body rotational diffusion, can be expressed as a sum of five exponential terms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.