ArticleThe Journal of cell biology1984
Tubulin dynamics in cultured mammalian cells.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 195 papers.
What it found
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Who cites it
195 citing papers in PubMed, 492 citations in OpenAlex.
- A single-component optogenetic toolkit for reversible visualization and programmable control of microtubule dynamics.Cell reports methods · 2026Article
- Towards a systems-level view of the microtubule cytoskeleton and its functions in physiology and disease.Nature reviews. Molecular cell biology · 2026Review
- Physical effects of crowdant size and concentration on collective microtubule polymerization.Biophysical journal · 2025Article
- Microtubule regulation in cancer cells.Frontiers in cell and developmental biology · 2025Article
- Measurements of neurite extension and nucleokinesis in an iPSC-derived model system following microtubule perturbation.Molecular biology of the cell · 2025Article
- AlphaFold2-guided engineering of split-GFP technology enables labeling of endogenous tubulins across species while preserving function.PLoS biology · 2024Article
- Noscapine and Apoptosis in Breast and Other Cancers.International journal of molecular sciences · 2024Review
- The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Towards understanding centriole elimination.Open biology · 2023Review
- Mechanisms underlying spindle assembly and robustness.Nature reviews. Molecular cell biology · 2023Review
- Article
- The "Third Violin" in the Cytoskeleton Orchestra-The Role of Intermediate Filaments in the Endothelial Cell's Life.Biomedicines · 2022Review
- The mitotic spindle protein CKAP2 potently increases formation and stability of microtubules.eLife · 2022Article
- Review
- Recent Approaches to the Identification of Novel Microtubule-Targeting Agents.Frontiers in molecular biosciences · 2022Review
- Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells.Life science alliance · 2021Article
- K-fiber bundles in the mitotic spindle are mechanically reinforced by Kif15.Molecular biology of the cell · 2021Article
- Article
- Relevance and Regulation of Cell Density.Trends in cell biology · 2020Review
- A comparative analysis of methods to measure kinetochore-microtubule attachment stability.Methods in cell biology · 2020Article
135 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Bovine neurotubulin has been labeled with dichlorotriazinyl-aminofluorescein (DTAF-tubulin) and microinjected into cultured mammalian cells strains PTK1 and BSC. The fibrous, fluorescence patterns that developed in the microinjected cells were almost indistinguishable from the pattern of microtubules seen in the same cells by indirect immunofluorescence. DTAF-tubulin participated in the formation of all visible, microtubule-related structures at all cell cycle stages for at least 48 h after injection. Treatments of injected cells with Nocodazole or Taxol showed that DTAF-tubulin closely mimicked the behavior of endogenous tubulin. The rate at which microtubules incorporated DTAF-tubulin depended on the cell-cycle stage of the injected cell. Mitotic microtubules became fluorescent within seconds while interphase microtubules required minutes. Studies using fluorescence redistribution after photobleaching confirmed this apparent difference in tubulin dynamics between mitotic and interphase cells. The temporal patterns of redistribution included a rapid phase (approximately 3 s) that we attribute to diffusion of free DTAF-tubulin and a second, slower phase that seems to represent the exchange of bleached DTAF-tubulin in microtubules with free, unbleached DTAF-tubulin. Mean half times of redistribution were 18-fold shorter in mitotic cells than they were in interphase cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.