ArticleThe Journal of cell biology1984
Diffusion coefficient of fluorescein-labeled tubulin in the cytoplasm of embryonic cells of a sea urchin: video image analysis of fluorescence redistribution after photobleaching.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 119 citations in OpenAlex.
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- Microtubule Growth Rates Are Sensitive to Global and Local Changes in Microtubule Plus-End Density.Current biology : CB · 2020Article
- Geometric Asymmetry Induces Upper Limit of Mitotic Spindle Size.Biophysical journal · 2017Article
- Biochemical perturbations of the mitotic spindle in Xenopus extracts using a diffusion-based microfluidic assay.Biomicrofluidics · 2015Article
- Multisite phosphorylation of the NDC80 complex gradually tunes its microtubule-binding affinity.Molecular biology of the cell · 2015Article
- Organization of early frog embryos by chemical waves emanating from centrosomes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Enhancement of biological reactions on cell surfaces via macromolecular crowding.Nature communications · 2014Article
- Slow diffusion underlies alternation of fast and slow growth periods of microtubule assembly.TheScientificWorldJournal · 2014Article
- Simulating intravitreal injections in anatomically accurate models for rabbit, monkey, and human eyes.Pharmaceutical research · 2012Article
- From microscopy data to in silico environments for in vivo-oriented simulations.EURASIP journal on bioinformatics & systems biology · 2012Article
- Vibrational dynamics of bio- and nano-filaments in viscous solution subjected to ultrasound: implications for microtubules.European biophysics journal : EBJ · 2011Article
- Polyamine sharing between tubulin dimers favours microtubule nucleation and elongation via facilitated diffusion.PLoS computational biology · 2009Article
- Compartment volume influences microtubule dynamic instability: a model study.Biophysical journal · 2006Article
- Model for anaphase B: role of three mitotic motors in a switch from poleward flux to spindle elongation.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Microtubule self-organisation by reaction-diffusion processes causes collective transport and organisation of cellular particles.BMC cell biology · 2004Article
- Microtubule Dynamics may Embody a Stationary Bipolarity Forming Mechanism Related to the Prokaryotic Division Site Mechanism (Pole-to-Pole Oscillations).Journal of biological physics · 2004Article
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5 authors.
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Abstract
The diffusion coefficient of tubulin has been measured in the cytoplasm of eggs and embryos of the sea urchin Lytechinus variegatus. We have used brain tubulin, conjugated to dichlorotriazinyl-aminofluorescein, to inject eggs and embryos. The resulting distributions of fluorescence were perturbed by bleaching with a microbeam of light from the 488-nm line of an argon ion laser. Fluorescence redistribution after photobleaching was monitored with a sensitive video camera and photography of the television-generated image. With standard photometric methods, we have calibrated this recording system and measured the rates of fluorescence redistribution for tubulin, conjugated to dichlorotriazinyl-aminofluorescein, not incorporated into the mitotic spindle. The diffusion coefficient (D) was calculated from these data using Fick's second law of diffusion and a digital method for analysis of the photometric curves. We have tested our method by determining D for bovine serum albumin (BSA) under conditions where the value is already known and by measuring D for fluorescein-labeled BSA in sea urchin eggs with a standard apparatus for monitoring fluorescence redistribution after photobleaching. The values agree to within experimental error. Dcytoplasmtubulin = 5.9 +/- 2.2 X 10(-8) cm2/s; DcytoplasmBSA = 8.6 +/- 2.0 X 10(-8) cm2/s. Because DH2OBSA = 68 X 10(-8) cm2/s, these data suggest that the viscosity of sea urchin cytoplasm for protein is about eight times that of water and that most of the tubulin of the sea urchin cytoplasm exists as a dimer or small oligomer, which is unbound to structures that would impede its diffusion. Values and limitations of our method are discussed, and we draw attention to both the variations in D for single proteins in different cells and the importance of D for the upper limit to the rates of polymerization reactions.
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