ArticleThe Journal of cell biology1976
The contractile basis of ameboid movement. II. Structure and contractility of motile extracts and plasmalemma-ectoplasm ghosts.
Article in The Journal of cell biology, 1976. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 73 citations in OpenAlex.
- Ca2+-sensitive isolation of a cortical actin matrix from Dictyostelium amoebae.Journal of muscle research and cell motility · 1983Article
- Mobility of cytoplasmic and membrane-associated actin in living cells.Proceedings of the National Academy of Sciences of the United States of America · 1982Article
- Isolation and characterization of actin and actin-binding protein from human platelets.The Journal of cell biology · 1981Article
- Characterization of antibodies to smooth muscle myosin kinase and their use in localizing myosin kinase in nonmuscle cells.Proceedings of the National Academy of Sciences of the United States of America · 1981Article
- A membrane cytoskeleton from Dictyostelium discoideum. I. Identification and partial characterization of an actin-binding activity.The Journal of cell biology · 1981Article
- Microfilaments and tropomyosin of cultured mammalian cells: isolation and characterization.The Journal of cell biology · 1980Article
- Localization of cytoplasmic and skeletal myosins in developing muscle cells by double-label immunofluorescence.The Journal of cell biology · 1980Article
- Cytoskeleton of human platelets at rest and after spreading.The Journal of cell biology · 1980Article
- Contractile basis of ameboid movement. VII. Aequorin luminescence during ameboid movement, endocytosis, and capping.The Journal of cell biology · 1980Article
- Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas.The Journal of cell biology · 1980Article
- Organization and spatial arrangement of fluorescein-labeled native actin microinjected into normal locomoting and experimentally influenced Amoeba proteus.Cell and tissue research · 1980Article
- The contractile basis of ameboid movement. VI. The solation-contraction coupling hypothesis.The Journal of cell biology · 1979Article
- pH regulates the polymerization of actin in the sea urchin egg cortex.The Journal of cell biology · 1979Article
- Changing patterns of plasma membrane-associated filaments during the initial phases of polymorphonuclear leukocyte adherence.The Journal of cell biology · 1979Article
- Effects of the actin-binding protein DNAase I on cytoplasmic streaming and ultrastructure of Amoeba proteus. An attempt to explain amoeboid movement.Cell and tissue research · 1979Article
- The role of actin in temperature-dependent gel-sol transformation of extracts of Ehrlich ascites tumor cells.The Journal of cell biology · 1979Article
- Chick brain actin and myosin. Isolation and characterization.The Journal of cell biology · 1979Article
- Development of the structural components of the brush border in absorptive cells of the chick intestine.Cell and tissue research · 1979Article
- Actin in Xenopus oocytes.The Journal of cell biology · 1978Article
- Molecular cytochemistry: incorporation of fluorescently labeled actin into living cells.Proceedings of the National Academy of Sciences of the United States of America · 1978Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of calcium and magnesium-ATP on the structure and contractility in motile extracts of Amoeba proteus and plasmalemma-ectoplasm "ghosts" of Chaos carolinensis has been investigated by correlating light and electron microscope observations with turbidity and birefringence measurements. The extract is nonmotile and contains very few F-actin filaments and myosin aggregates when prepared in the presence of both low calcium ion and ATP concentrations at an ionic strength of I = 0.05, pH 6.8. The addition of 1.0 mM magnesium chloride, 1.0 mM ATP, in the presence of a low calcium ion concentration (relaxation solution) induced the formation of some fibrous bundles of actin without contracting, whereas the addition of a micromolar concentration of calcium in addition to 1.0 mM magnesium-ATP (contraction solution) (Taylor, D. L., J. S. Condeelis, P. L. Moore, and R. D. Allen. 1973. J. Cell Biol. 59:378-394) initiated the formation of large arrays of F-actin filaments followed by contractions. Furthermore, plasmalemma-ectoplasm ghosts prepared in the relaxation solution exhibited very few straight F-actin filaments and myosin aggregates. In contrast, plasmalemmaectoplasm ghosts treated with the contraction solution contained many straight F-actin filaments and myosin aggregates. The increase in the structure of ameba cytoplasm at the endoplasm-ectoplasm interface can be explained by a combination of the transformation of actin from a less filamentous to a more structured filamentous state possibly involving the cross-linking of actin to form fibrillar arrays (see above-mentioned reference) followed by contractions of the actin and myosin along an undetermined distance of the endoplasm and/or ectoplasm.
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