Evidence map›Paper›PMID 6438113›Full record

ArticleThe Journal of cell biology1984

Assembly properties of fluorescein-labeled tubulin in vitro before and after fluorescence bleaching.

R J Leslie, W M Saxton, T J Mitchison, B Neighbors, E D Salmon, J R McIntosh

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PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 59 citations in OpenAlex.

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  10. Contribution of midbody channels to embryogenesis in the mouse : Analysis by immunofluorescence.Roux's archives of developmental biology : the official organ of the EDBO · 1988
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  17. Tubulin dynamics in cultured mammalian cells.The Journal of cell biology · 1984
    Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

R J Leslie
W M Saxton
T J Mitchison
B Neighbors
E D Salmon
J R McIntosh

Funding

NIGMS NIH HHS GM-31213
6 · The paper itself

Abstract

Brain tubulin has been conjugated with dichlorotriazinyl-aminofluorescein (DTAF) to form a visualizable complex for the study of tubulin dynamics in living cells. By using several assays we confirm the finding of Keith et al. (Keith, C. H., J. R. Feramisco, and M. Shelanski, 1981, J. Cell Biol., 88:234-240) that DTAF-tubulin polymerizes like control tubulin in vitro. The fluorescein moiety of the complex is readily bleached by the 488-nm line from an argon ion laser. When irradiations are performed over short times (less than 1 s) and in the presence of 2 mM glutathione, a mixture of DTAF-tubulin and control protein (as occurs after microinjection of the fluorescent conjugate into living cells) will retain full polymerization activity. Slow bleaching (approximately 5 min) or bleaching without glutathione promotes formation of covalent cross-links between neighboring polypeptides and kills the polymerization activity of DTAF-tubulin, including some molecules that are neither cross-linked nor bleached. Even under conditions that damage DTAF-tubulin, however, DTAF-microtubules are not destroyed by bleaching. They will continue to elongate by addition of DTAF-tubulin subunits to their free ends, and they neither bind nor exchange subunits along their lateral surfaces. These results suggest that DTAF-tubulin is a suitable analog for tubulin, both in studies of protein incorporation and for investigations of fluorescence redistribution after photobleaching.

Indexed as

AnimalsBrainCattleFlagellaFluorescein-5-isothiocyanateFluoresceinsFluorescent DyesKineticsLasersMacromolecular SubstancesMicrotubulesSea UrchinsThiocyanatesTubulin5-((4,6-dichloro-1,3,5-triazin-2-yl)amino)fluoresceinFluorescein-5-isothiocyanateFluoresceinsFluorescent DyesMacromolecular SubstancesThiocyanatesTubulin

Identifiers

PMID6438113
PMCPMC2113541
OpenAlexW2105404184

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.