ArticleThe Journal of cell biology1983
High-resolution three-dimensional views of membrane-associated clathrin and cytoskeleton in critical-point-dried macrophages.
Article in The Journal of cell biology, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Structural Diversity within the Endoplasmic Reticulum-From the Microscale to the Nanoscale.Cold Spring Harbor perspectives in biology · 2023Review
- Article
- Imaging endocytic clathrin structures in living cells.Trends in cell biology · 2009Review
- Unrestricted diffusion of exogenous and endogenous PIP(2 )in baby hamster kidney and Chinese hamster ovary cell plasmalemma.The Journal of membrane biology · 2007Article
- Local PIP(2) signals: when, where, and how?Pflugers Archiv : European journal of physiology · 2007Review
- The role of clathrin-dependent endocytosis in bacterial internalization.Trends in cell biology · 2006Review
- Agonist-induced endocytosis of CC chemokine receptor 5 is clathrin dependent.Molecular biology of the cell · 2005Article
- Barriers for lateral diffusion of transferrin receptor in the plasma membrane as characterized by receptor dragging by laser tweezers: fence versus tether.The Journal of cell biology · 1995Article
- Compartmentalized structure of the plasma membrane for receptor movements as revealed by a nanometer-level motion analysis.The Journal of cell biology · 1994Article
- Acetylcholine receptor clusters of rat myotubes have at least three domains with distinctive cytoskeletal and membranous components.The Journal of cell biology · 1989Article
- Rapid fragmentation and reorganization of Golgi membranes during frustrated phagocytosis of immobile immune complexes by macrophages.The American journal of pathology · 1989Article
- Three-dimensional organization of the extracellular matrix secreted by cultured rat smooth muscle cells.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1988Article
- Influence of a reconstituted basement membrane and its components on casein gene expression and secretion in mouse mammary epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 1987Article
- Potassium-dependent assembly of coated pits: new coated pits form as planar clathrin lattices.The Journal of cell biology · 1986Article
- Clathrin-coated vesicular transport of secretory proteins during the formation of ACTH-containing secretory granules in AtT20 cells.The Journal of cell biology · 1986Article
- Epidermal growth factor receptors associated to cytoskeletal elements of epidermoid carcinoma (A431) cells.The Journal of cell biology · 1986Article
- Rapid redistribution of clathrin onto macrophage plasma membranes in response to Fc receptor-ligand interaction during frustrated phagocytosis.The Journal of cell biology · 1986Article
- Organization of filaments underneath the plasma membrane of developing chicken skeletal muscle cells in vitro revealed by the freeze-dry and rotary replica method.Cell and tissue research · 1986Article
- Exocytosis of sea urchin egg cortical vesicles in vitro is retarded by hyperosmotic sucrose: kinetics of fusion monitored by quantitative light-scattering microscopy.The Journal of cell biology · 1985Article
- Endothelial plasmalemmal vesicles have a characteristic striped bipolar surface structure.The Journal of cell biology · 1985Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We obtained high-resolution topographical information about the distribution of clathrin and cytoskeletal filaments on cytoplasmic membrane surfaces of macrophages spreading onto glass coverslips by both critical-point drying of broken-open cells and preparation of rotary platinum replicas. Irregular patches of the adherent ventral surface of the plasma membrane were exposed in these cells, and large areas of these exposed membranes were covered with clathrin-coated patches, pits, and vesicles. Various amounts of cytoskeleton were attached to the plasma membranes of these spreading cells, either as distinct starlike foci, or as individual filaments and bundles radiating out from the cytoskeletal meshwork. In newly adherent cells a well developed Golgi-GERL complex, characterized by smooth, dish-like cisternae associated with rough endoplasmic reticulum, was observed. There were many coated vesicles budding off from the Golgi cisternae, and these were predominantly of the large type (150 nm) usually associated with the plasma membrane. In critical-point-dried samples, both cytoskeleton and membranes were preserved in detail comparable to that of quick-frozen samples, after appropriate fixation. Rotary replication of critical-point-dried cells provides a rapid, easily controlled, and generally easy to perform method for obtaining samples of exposed membrane large enough to permit quantification of membrane-associated clathrin and cytoskeleton under various experimental conditions.
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