ArticleProceedings of the National Academy of Sciences of the United States of America1984
The translational mobility of substances within the cytoplasmic matrix.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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56 citing papers in PubMed, 155 citations in OpenAlex.
- What's past is prologue: FRAP keeps delivering 50 years later.Biophysical journal · 2023Review
- Stochastic model of ERK-mediated progesterone receptor translocation, clustering and transcriptional activity.Scientific reports · 2022Article
- Intracellular transport dynamics revealed by single-particle tracking.Biophysics reports · 2021Article
- Non-specific interactions govern cytosolic diffusion of nanosized objects in mammalian cells.Nature materials · 2018Article
- Cellular automaton-based model for radiation-induced bystander effects.BMC systems biology · 2015Article
- Kinetic Monte Carlo simulations of the assembly of filamentous biomacromolecules by dimer addition mechanism.RSC advances · 2015Article
- Quantitative imaging of cerebral blood flow velocity and intracellular motility using dynamic light scattering-optical coherence tomography.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2013Article
- Osmostress-induced cell volume loss delays yeast Hog1 signaling by limiting diffusion processes and by Hog1-specific effects.PloS one · 2013Article
- Spatial simulations in systems biology: from molecules to cells.International journal of molecular sciences · 2012Review
- A simulation study of the radiation-induced bystander effect: modeling with stochastically defined signal reemission.Computational and mathematical methods in medicine · 2012Article
- Gelsolin and non-muscle myosin IIA interact to mediate calcium-regulated collagen phagocytosis.The Journal of biological chemistry · 2011Article
- Heterogeneity in the kinetics of nuclear proteins and trajectories of substructures associated with heterochromatin.Epigenetics & chromatin · 2011Article
- Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange.ACS nano · 2010Article
- Routes of epithelial water flow: aquaporins versus cotransporters.Biophysical journal · 2010Article
- Visualizing dynamic interaction between calmodulin and calmodulin-related kinases via a monitoring method in live mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Effects of macromolecular crowding on intracellular diffusion from a single particle perspective.Biophysical reviews · 2010Article
- Stochastic simulation of signal transduction: impact of the cellular architecture on diffusion.Biophysical journal · 2009Article
- Model for Protein Concentration Gradients in the Cytoplasm.Cellular and molecular bioengineering · 2008Article
- Resonant waveguide grating biosensor for living cell sensing.Biophysical journal · 2006Article
- Stochastic model of protein-protein interaction: why signaling proteins need to be colocalized.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
Corrections and comments
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The translational mobility of fluorescent-labeled molecules injected into the cytoplasm of living cells can be measured by the fluorescence recovery after photobleaching (FRAP) technique. In the fibroblast cytoplasm, the diffusion coefficients, D, of test macromolecules ranging in molecular weight from 12,000 to 440,000 are about 10(-8) cm2/sec and exhibit almost no dependence on molecular weight. FRAP experiments also showed that macromolecular diffusion within Sepharose beads having an effective pore size smaller than the "microtrabecular lattice" is only slightly retarded compared to buffer values--in contrast to the marked retardation measured in the cytoplasm. This leads to the conclusion that diffusion in the cytomatrix is dominated not by steric effects but rather by binding of the diffusing species to elements of the cytomatrix. These diffusion rates were difficult to modulate; cytochalasin, colchicine (except at 5 degrees C), and taxol treatments had little effect. The diffusion rates were not dependent on cellular energy metabolism. However, hypotonic treatment increased the D for bovine serum albumin by nearly 2-fold, whereas hypertonic treatment halved D. Withdrawing the free water from the cell by using 44% polyethylene glycol treatment stopped the translational mobility of the test molecules. A survey of the recent literature is presented, which shows that major differences in the cytomatrix of different cell types exist with respect to the translational diffusion of injected probes. Finally, the spectrum of cytoplasmic translational mobilities ranging from small molecules to organelles is discussed.
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