ArticleThe Journal of cell biology1984
Localization of actin and myosin for the study of ameboid movement in Dictyostelium using improved immunofluorescence.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers.
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Who cites it
107 citing papers in PubMed.
- Actomyosin dynamics in detached cells: linking clutch model to cell migration and cytokinesis.BMC molecular and cell biology · 2025Article
- Yeast with elevated chromosome numbers are addicted to high levels of Mps1.bioRxiv : the preprint server for biology · 2025Article
- Multiple Mechanisms to Regulate Actin Functions: "Fundamental" Versus Lineage-Specific Mechanisms and Hierarchical Relationships.Biomolecules · 2025Review
- Microtubule dependent sorting of actin-binding proteins in mitosis.Scientific reports · 2024Article
- Wound Repair of the Cell Membrane: Lessons fromCells · 2024Review
- Patterning of the cell cortex and the localization of cleavage furrows in multi-nucleate cells.Journal of cell science · 2022Article
- Deletion of gmfA induces keratocyte-like migration in Dictyostelium.FEBS open bio · 2022Article
- A 'dynamic adder model' for cell size homeostasis in Dictyostelium cells.Scientific reports · 2021Article
- Electric signals counterbalanced posterior vs anterior PTEN signaling in directed migration of Dictyostelium.Cell & bioscience · 2021Article
- Article
- Cell Mechanics at the Rear Act to Steer the Direction of Cell Migration.Cell systems · 2020Article
- Cleavage-furrow formation without F-actin inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Long-Range and Directional Allostery of Actin Filaments Plays Important Roles in Various Cellular Activities.International journal of molecular sciences · 2020Review
- CaCells · 2020Review
- Extranuclear Structural Components that Mediate Dynamic Chromosome Movements in Yeast Meiosis.Current biology : CB · 2020Article
- Pressure sensing through Piezo channels controls whether cells migrate with blebs or pseudopods.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Regulation of the Total Cell Surface Area in DividingFrontiers in cell and developmental biology · 2020Article
- Tree of motility - A proposed history of motility systems in the tree of life.Genes to cells : devoted to molecular & cellular mechanisms · 2020Review
- Proteomic and Transcriptomic Profiling Identifies Early Developmentally Regulated Proteins inCells · 2019Article
47 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The distribution of actin and myosin in Dictyostelium amebae at different developmental stages was studied by improved immunofluorescence ("agar-overlay" technique). Both were localized at the cortical region of amebae in all early developmental stages. In amebae with polarized morphology, bright fluorescence with antiactin was seen in the anterior pseudopode. The cortex in the posterior end was also stained with antiactin. On the other hand, very specific crescent-shaped staining with antimyosin was seen at the posterior cortex. In cells in contact with each other, actin was concentrated at the contact region, whereas myosin was localized specifically in the cortex on the other side of the contact region. At the aggregation stage, when monopodial amebae migrate forming streams, actin staining was seen all around the cell periphery, with intense fluorescence in the anterior pseudopode. On the other hand, specific staining of myosin was seen only at the posterior cortex. The cleavage furrow of cells performing cytokinesis displayed distinct myosin staining, and this staining represented the filamentous structure aligned in parallel to the axis of constriction. These findings indicate that myosin staining reflects the portion of the cell cortex where contraction occurs and the motive force of ameboid movement is generated at the posterior cortex of a migrating cell.
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