ArticleProceedings of the National Academy of Sciences of the United States of America1984
Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 178 citations in OpenAlex.
- Yeast Tools for Studying Type II Topoisomerases in Budding Yeast.Methods in molecular biology (Clifton, N.J.) · 2025Article
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- Age-Onset Phosphorylation of a Minor Actin Variant Promotes Intestinal Barrier Dysfunction.Developmental cell · 2019Article
- Unintended Side Effects of Transformation Are Very Rare inG3 (Bethesda, Md.) · 2018Article
- Development of a Comprehensive Genotype-to-Fitness Map of Adaptation-Driving Mutations in Yeast.Cell · 2016Article
- Article
- Evidence for monomeric actin function in INO80 chromatin remodeling.Nature structural & molecular biology · 2013Article
- Nuclear dynamics, mitosis, and the cytoskeleton during the early stages of colony initiation in Neurospora crassa.Eukaryotic cell · 2010Article
- F-actin dynamics in Neurospora crassa.Eukaryotic cell · 2010Article
- Rapid screening for temperature-sensitive alleles in plants.Plant physiology · 2009Article
- Article
- A high-efficiency method to replace essential genes with mutant alleles in yeast.Yeast (Chichester, England) · 2005Article
- Random mutagenesis of an essential Candida albicans gene.Current genetics · 2004Article
- Suppressor mutations define two regions in the Cbp1 protein important for mitochondrial cytochrome b mRNA stability in Saccharomyces cerevisiae.Current genetics · 2003Article
- Overexpression of translation elongation factor 1A affects the organization and function of the actin cytoskeleton in yeast.Genetics · 2001Article
- Generation of an isogenic collection of yeast actin mutants and identification of three interrelated phenotypes.Genetics · 2001Article
- Article
- Uracil-induced down-regulation of the yeast uracil permease.Journal of bacteriology · 1999Article
- Mlc1p is a light chain for the unconventional myosin Myo2p in Saccharomyces cerevisiae.The Journal of cell biology · 1998Article
15 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Two temperature-sensitive mutations have been constructed in the single actin gene (ACT1) of the yeast Saccharomyces cerevisiae by in vitro mutagenesis of the cloned gene followed by integrative transformation of mutagenized DNA into yeast cells. A strategy of allele replacement was used that allowed recessive mutations to be phenotypically expressed in the initial transformants, thus simplifying the screening of large numbers of independently transformed cells. After confirming that several ts mutations were located within the actin structural gene by genetic methods, these mutant alleles were cloned, and the altered amino acid residues were defined by DNA sequence analysis. The two unique mutations resulted in substitution of proline-32 with leucine and alanine-58 with threonine. In the course of isolating these mutations, the observation was made that a high proportion of yeast cells transformed with exogenous DNA by the spheroplast method are temperature sensitive for growth because of genetic changes unrelated to the transforming DNA.
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