ArticleThe Journal of cell biology1984
Microinjected fluorescent polystyrene beads exhibit saltatory motion in tissue culture cells.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 54 citations in OpenAlex.
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Probing and manipulating intracellular membrane traffic by microinjection of artificial vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Flexibility contra stiffness: the phragmoplast as a physical barrier for beads but not for vesicles.Plant physiology · 2010Article
- Micromechanical mapping of live cells by multiple-particle-tracking microrheology.Biophysical journal · 2002Article
- Viscoelastic response of fibroblasts to tension transmitted through adherens junctions.Biophysical journal · 1997Article
- Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment.Biophysical journal · 1995Article
- Zyxin and cCRP: two interactive LIM domain proteins associated with the cytoskeleton.The Journal of cell biology · 1992Article
- An interaction between zyxin and alpha-actinin.The Journal of cell biology · 1992Article
- Magnetic particle motions within living cells. Measurement of cytoplasmic viscosity and motile activity.Biophysical journal · 1987Article
- Translocation and clustering of endosomes and lysosomes depends on microtubules.The Journal of cell biology · 1987Article
- Rabies virus infection of cultured rat sensory neurons.Journal of virology · 1987Article
- Motion of mitochondria in cultured cells quantified by analysis of digitized images.Biophysical journal · 1985Article
- Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.Cell · 1985Article
- Fast axonal transport of foreign synaptic vesicles in squid axoplasm.The Journal of cell biology · 1985Article
- Gliding movement of and bidirectional transport along single native microtubules from squid axoplasm: evidence for an active role of microtubules in cytoplasmic transport.The Journal of cell biology · 1985Article
- The translational mobility of substances within the cytoplasmic matrix.Proceedings of the National Academy of Sciences of the United States of America · 1984Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microinjected 0.26-micron fluorescent, carboxylated microspheres were found to display classical saltatory motion in tissue culture cells. The movement of a given particle was characterized by a discontinuous velocity distribution and was unaffected by the activity of adjacent particles. The microspheres were translocated at velocities of up to 4.7 micron/s and sometimes exhibited path lengths greater than 20 micron for a single saltation . The number of beads injected into a cell could range from a few to over 500 with no effect on the cell's ability to transport them. Neither covalent cross-linking nor preincubation of the polystyrene beads with various proteins inhibited the saltatory motion of the injected particles. The motion of the injected beads in cultured cells was reversibly inhibited by the microtubule poison nocodazole, under conditions in which actin-rich, nitrobenzoxadiazol - phallacidin -staining structures remain intact. Whole-cell high voltage electron microscopy of microinjected cells that were known to be moving the fluorescent microspheres revealed that the beads were embedded in the cytoplasmic matrix and did not appear to be membrane bound. The enhanced detectability of the fluorescent particles over endogenous organelles and the ability to modify the surfaces of the beads before injection may enable more detailed studies on the mechanism of saltatory particle motion.
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