ArticleThe Journal of cell biology1984
Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 388 papers.
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Who cites it
388 citing papers in PubMed, 825 citations in OpenAlex.
- Organelle partitioning in the multi-budding yeastbioRxiv : the preprint server for biology · 2026Article
- Localization of Proteins in the Secretory Pathway of Pichia pastoris Using Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A genetic strategy to allow detection of F-actin by phalloidin staining in diverse fungi.mSphere · 2025Article
- The conserved protein adaptors CALM/AP180 and FCHo1/2 cooperatively recruit Eps15 to promote the initiation of clathrin-mediated endocytosis in yeast.PLoS biology · 2024Article
- Improved tools for live imaging of F-actin structures in yeast.Molecular biology of the cell · 2024Article
- Yeast Rim11 kinase responds to glutathione-induced stress by regulating the transcription of phospholipid biosynthetic genes.Molecular biology of the cell · 2024Article
- Purification of Human Cytoplasmic Actins FromBio-protocol · 2023Article
- A dynamic actin cytoskeleton is required to prevent constitutive VDAC-dependent MAPK signalling and aberrant lipid homeostasis.iScience · 2023Article
- Tubulin isotype regulation maintains asymmetric requirement for α-tubulin over β-tubulin.The Journal of cell biology · 2023Article
- Going with the membrane flow: the impact of polarized secretion on bulk plasma membrane flows.The FEBS journal · 2023Review
- Actin-bundling protein fimbrin regulates pathogenicity via organizing F-actin dynamics during appressorium development in Colletotrichum gloeosporioides.Molecular plant pathology · 2022Article
- Article
- Selective Inhibition of Plasmodium falciparum ATPase 6 by Artemisinins and Identification of New Classes of Inhibitors after Expression in Yeast.Antimicrobial agents and chemotherapy · 2022Article
- NADPH Oxidases Play a Role in PathogenicityFrontiers in cellular and infection microbiology · 2022Article
- Ultrastructural analysis in yeast reveals a meiosis-specific actin-containing nuclear bundle.Communications biology · 2021Article
- Selection and Validation of Reference Genes for qRT-PCR Analysis in the Oil-Rich Tuber Crop Tiger Nut (International journal of molecular sciences · 2021Article
- The mitotic exit network regulates the spatiotemporal activity of Cdc42 to maintain cell size.The Journal of cell biology · 2021Article
- Yeast Sphingolipid Phospholipase GeneMolecular and cellular biology · 2020Article
- Review
- The elusive actin cytoskeleton of a green alga expressing both conventional and divergent actins.Molecular biology of the cell · 2019Article
328 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The distribution of actin and tubulin during the cell cycle of the budding yeast Saccharomyces was mapped by immunofluorescence using fixed cells from which the walls had been removed by digestion. The intranuclear mitotic spindle was shown clearly by staining with a monoclonal antitubulin; the presence of extensive bundles of cytoplasmic microtubules is reported. In cells containing short spindles still entirely within the mother cells, one of the bundles of cytoplasmic microtubules nearly always extended to (or into) the bud. Two independent reagents (anti-yeast actin and fluorescent phalloidin) revealed an unusual distribution of actin: it was present as a set of cortical dots or patches and also as distinct fibers that were presumably bundles of actin filaments. Double labeling showed that at no stage in the cell cycle do the distributions of actin and tubulin coincide for any significant length, and, in particular, that the mitotic spindle did not stain detectably for actin. However, both microtubule and actin staining patterns change in a characteristic way during the cell cycle. In particular, the actin dots clustered in rings about the bases of very small buds and at the sites on unbudded cells at which bud emergence was apparently imminent. Later in the budding cycle, the actin dots were present largely in the buds and, in many strains, primarily at the tips of these buds. At about the time of cytokinesis the actin dots clustered in the neck region between the separating cells. These aspects of actin distribution suggest that it may have a role in the localized deposition of new cell wall material.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.