ArticleThe Journal of cell biology1983
Antibodies to a nucleolar protein are localized in the nucleolus after red blood cell-mediated microinjection.
Article in The Journal of cell biology, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 20 citations in OpenAlex.
- Coilin shuttles between the nucleus and cytoplasm in Xenopus oocytes.Molecular biology of the cell · 1999Article
- Injection of anticentromere antibodies in interphase disrupts events required for chromosome movement at mitosis.The Journal of cell biology · 1990Article
- Antiviral state against influenza virus neutralized by microinjection of antibodies to interferon-induced Mx proteins.The EMBO journal · 1988Article
- Microinjected glutathione S-transferase Yb subunits translocate to the cell nucleus.The Biochemical journal · 1987Article
- Monoclonal antibody specific for human nuclear proteins IEF 8Z30 and 8Z31 accumulates in the nucleus a few hours after cytoplasmic microinjection of cells expressing these proteins.The Journal of cell biology · 1986Article
- Epitope distribution and immunochemical characterization of nucleolar phosphoprotein C23 using ten monoclonal antibodies.Molecular and cellular biochemistry · 1985Article
- An efficient method for introducing macromolecules into living cells.The Journal of cell biology · 1985Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
To determine whether red blood cell-mediated microinjection of antibodies can be used to study nuclear protein localization and function, we microinjected antibodies that have been shown to react specifically with nucleolar acidic phosphoprotein C23 into Walker 256 cells. The intracellular distribution of microinjected anti-C23 antibodies and preimmune immunoglobulins were determined by immunofluorescence. At 3 h after microinjection, affinity-purified anti-C23 antibodies were localized in the cytoplasm and nucleolus. At 17 h after microinjection, the affinity-purified antibody was localized to those nucleolar structures previously shown to contain protein C23. Furthermore, the antibody remained localized in the nucleolus for at least 36 h after microinjection. In contrast to the results obtained with specific antibodies, preimmune immunoglobulins remained in the cytoplasm 36 h after microinjection. These results indicate that red blood cell-mediated microinjection of antibodies can be used to study nucleolar and nuclear antigens.
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Registered trials
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