ArticleThe Journal of cell biology1983
Differences in the organization of actin in the growth cones compared with the neurites of cultured neurons from chick embryos.
Article in The Journal of cell biology, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 210 citations in OpenAlex.
- The TOG5 domain of CKAP5 is required to interact with F-actin and promote microtubule advancement in neurons.Molecular biology of the cell · 2024Article
- TIAM-1 differentially regulates dendritic and axonal microtubule organization in patterning neuronal development through its multiple domains.PLoS genetics · 2022Article
- Neurons: The Interplay between Cytoskeleton, Ion Channels/Transporters and Mitochondria.Cells · 2022Review
- p53 Related Protein Kinase is Required for Arp2/3-Dependent Actin Dynamics of Hemocytes inFrontiers in cell and developmental biology · 2022Article
- EFA6 in Axon Regeneration, as a Microtubule Regulator and as a Guanine Nucleotide Exchange Factor.Cells · 2021Review
- With the Permission of Microtubules: An Updated Overview on Microtubule Function During Axon Pathfinding.Frontiers in molecular neuroscience · 2021Review
- Microtubules: Evolving roles and critical cellular interactions.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- XMAP215 promotes microtubule-F-actin interactions to regulate growth cone microtubules during axon guidance inJournal of cell science · 2019Article
- The nano-architecture of the axonal cytoskeleton.Nature reviews. Neuroscience · 2017Review
- Cytoskeletal social networking in the growth cone: How +TIPs mediate microtubule-actin cross-linking to drive axon outgrowth and guidance.Cytoskeleton (Hoboken, N.J.) · 2016Review
- Bidirectional actin transport is influenced by microtubule and actin stability.Cellular and molecular life sciences : CMLS · 2015Article
- Actin dynamics in growth cone motility and navigation.Journal of neurochemistry · 2014Review
- Growth cone-specific functions of XMAP215 in restricting microtubule dynamics and promoting axonal outgrowth.Neural development · 2013Article
- Autocrine signaling based selection of combinatorial antibodies that transdifferentiate human stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- TAG1 regulates the endocytic trafficking and signaling of the semaphorin3A receptor complex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Mechanisms of neuronal growth cone guidance: an historical perspective.Developmental neurobiology · 2011Article
- Topography and nanomechanics of live neuronal growth cones analyzed by atomic force microscopy.Biophysical journal · 2009Article
- The trip of the tip: understanding the growth cone machinery.Nature reviews. Molecular cell biology · 2009Review
- The actin cross-linking protein AFAP120 regulates axon elongation in a tyrosine phosphorylation-dependent manner.Neuroscience letters · 2008Article
- RhoA-kinase coordinates F-actin organization and myosin II activity during semaphorin-3A-induced axon retraction.Journal of cell science · 2006Article
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sensory neurons from chick embryos were cultured on substrata that support neurite growth, and were fixed and prepared for both cytochemical localization of actin and electron microscopic observation of actin filaments in whole-mounted specimens. Samples of cells were treated with the detergent Triton X-100 before, during, or after fixation with glutaraldehyde to determine the organization of actin in simpler preparations of extracted cytoskeletons. Antibodies to actin and a fluorescent derivative of phallacidin bound strongly to the leading margins of growth cones, but in neurites the binding of these markers for actin was very weak. This was true in all cases of Triton X-100 treatment, even when cells were extracted for 4 min before fixation. In whole-mounted cytoskeletons there were bundles and networks of 6-7-nm filaments in leading edges of growth cones but very few 6-7-n filaments were present among the microtubules and neurofilaments in the cytoskeletons of neurites. These filaments, which are prominent in growth cones, were identified as actin because they were stabilized against detergent extraction by the presence of phallacidin or the heavy meromyosin and S1 fragments of myosin. In addition, heavy meromyosin and S1 decorated these filaments as expected for binding to F-actin. Microtubules extended into growth cone margins and terminated within the network of actin filaments and bundles. Interactions between microtubule ends and these actin filaments may account for the frequently observed alignment of microtubules with filopodia at the growth cone margins.
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