ArticleThe Journal of cell biology1984
Detection of single microtubules in living cells: particle transport can occur in both directions along the same microtubule.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 110 citations in OpenAlex.
- Methods to Measure Water Permeability.Advances in experimental medicine and biology · 2023Article
- Moving into the cell: single-molecule studies of molecular motors in complex environments.Nature reviews. Molecular cell biology · 2011Review
- Overview of image analysis, image importing, and image processing using freeware.Current protocols in molecular biology · 2003Article
- Organelle-cytoskeletal interactions: actin mutations inhibit meiosis-dependent mitochondrial rearrangement in the budding yeast Saccharomyces cerevisiae.Molecular biology of the cell · 1995Article
- Motility of bile canaliculi in the living animal: implications for bile flow.The Journal of cell biology · 1991Article
- Regulation of organelle transport in melanophores by calcineurin.The Journal of cell biology · 1990Article
- Monoclonal antibodies to kinesin heavy and light chains stain vesicle-like structures, but not microtubules, in cultured cells.The Journal of cell biology · 1989Article
- Real-time observations of microtubule dynamic instability in living cells.The Journal of cell biology · 1988Article
- The unusual microtubule polarity in teleost retinal pigment epithelial cells.The Journal of cell biology · 1988Article
- A survey of the alpha-tubulin gene family in chicken: unexpected sequence heterogeneity in the polypeptides encoded by five expressed genes.The EMBO journal · 1988Article
- Modification of the cell surface expression of histocompatibility antigens induced by the neurotoxin 2,5 hexanedione.Cell biology and toxicology · 1987Article
- Movement of mitochondria in the ovarian trophic cord of Dysdercus intermedius (Heteroptera) resembles nerve axonal transport.Roux's archives of developmental biology : the official organ of the EDBO · 1987Article
- In vivo microtubules are copolymers of available beta-tubulin isotypes: localization of each of six vertebrate beta-tubulin isotypes using polyclonal antibodies elicited by synthetic peptide antigens.The Journal of cell biology · 1987Article
- Dynamic shape changes of cytoplasmic organelles translocating along microtubules.The Journal of cell biology · 1987Article
- Reactivated melanophore motility: differential regulation and nucleotide requirements of bidirectional pigment granule transport.The Journal of cell biology · 1986Article
- Sequence and expression of the chicken beta 5- and beta 4-tubulin genes define a pair of divergent beta-tubulins with complementary patterns of expression.Molecular and cellular biology · 1986Article
- Microtubule polarity confers direction to pigment transport in chromatophores.The Journal of cell biology · 1986Article
- Identification of conserved isotype-defining variable region sequences for four vertebrate beta tubulin polypeptide classes.Proceedings of the National Academy of Sciences of the United States of America · 1986Article
- Cell shape and motility of oligodendrocytes cultured without neurons.Cell and tissue research · 1986Article
- Apparent gene conversion between beta-tubulin genes yields multiple regulatory pathways for a single beta-tubulin polypeptide isotype.Molecular and cellular biology · 1985Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Video-enhanced contrast/differential interference-contrast microscopy was used in conjunction with whole mount electron microscopy to study particle transport along linear elements in fibroblasts. Keratocytes from the corneal stroma of Rana pipiens were grown on gold indicator grids and examined with video microscopy. Video records were taken of the linear elements and associated particle transport until lysis and/or fixation of the cells was completed. The preparations were then processed for whole mount electron microscopy. By combining these two methods, we demonstrated that linear elements detected in the living cell could be identified as single microtubules, and that filaments as small as 10 nm could be detected in lysed and fixed cells. The visibility of different cytoplasmic structures changed after lysis with many more cellular components becoming visible. Microtubules became more difficult to detect after lysis while bundles of microfilaments became more prominent. All particle translocations were observed to take place along linear elements composed of one or more microtubules. Furthermore, particles were observed to translocate in one or both directions on the same microtubule.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.