ArticleThe Journal of cell biology1983
Transformation-sensitive isoactin in passaged chick embryo fibroblasts transformed by Rous sarcoma virus.
Article in The Journal of cell biology, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 41 citations in OpenAlex.
- High level expression of transfected beta- and gamma-actin genes differentially impacts on myoblast cytoarchitecture.The Journal of cell biology · 1992Article
- Expression of smooth muscle cell phenotype by rat mesangial cells in immune complex nephritis. Alpha-smooth muscle actin is a marker of mesangial cell proliferation.The Journal of clinical investigation · 1991Article
- Transformation of rabbit vascular smooth muscle cells by transfection with the early region of SV40 DNA.The American journal of pathology · 1990Article
- Altered expression of a third actin accompanying malignant progression in mouse B16 melanoma cells.Japanese journal of cancer research : Gann · 1989Article
- Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts.Molecular and cellular biology · 1988Article
- A molecular defect in virally transformed muscle cells that cannot cluster acetylcholine receptors.The Journal of cell biology · 1988Article
- Expression of transfected mutant beta-actin genes: transitions toward the stable tumorigenic state.Molecular and cellular biology · 1987Article
- Repression of quiescence-specific polypeptides in chicken heart mesenchymal cells transformed by Rous sarcoma virus.Molecular and cellular biology · 1987Article
- A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.The Journal of cell biology · 1986Article
- Immunolocalization of the gamma isoform of nonmuscle actin in cultured cells.The Journal of cell biology · 1986Article
- Increase in actin contents and elongation of apical projections in retinal pigmented epithelial cells during development of the chicken eye.The Journal of cell biology · 1985Article
- Altered beta-actin gene expression in phorbol myristate acetate-treated chondrocytes and fibroblasts.Molecular and cellular biology · 1985Article
- Differential control of cytokeratins and vimentin synthesis by cell-cell contact and cell spreading in cultured epithelial cells.The Journal of cell biology · 1984Article
- Multiple tropomyosin polypeptides in chicken embryo fibroblasts: differential repression of transcription by Rous sarcoma virus transformation.Molecular and cellular biology · 1984Article
- Actin expression in smooth muscle cells of rat aortic intimal thickening, human atheromatous plaque, and cultured rat aortic media.The Journal of clinical investigation · 1984Article
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3 authors.
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Abstract
Transformation by Rous sarcoma virus (RSV) has been reported to block the expression of differentiated cell products in chicken cells. The expression of these proteins may or may not be suppressed when temperature-sensitive mutants are shifted from the nonpermissive to the permissive temperature. A general characteristic of cellular transformation is the disruption of the microfilament system. In passaged chick embryo fibroblast cultures (CEF), this system is principally composed of isomeric forms of actin designated alpha, beta, and gamma by their isoelectric focusing and when subjected to SDS-PAGE behavior. We present evidence that an alpha-actin in CEF cultures, identified by its electrofocusing behavior, retention in the cytoskeleton, and DNase 1 binding properties, is selectively and dramatically reduced in amount upon transformation by RSV. Little or no reduction is observed in the beta- and gamma-isoactins. The reduction of alpha-actin is shown to be reversible and transformation related by use of a temperature-sensitive mutant, tsNY68. The decrease in this transformation-sensitive isoactin is apparently due to a decrease in synthesis, though other possibilities are discussed. A specific decrease in a particular isoactin after transformation may give insight into the mechanism by which the microfilaments are normally maintained.
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