ArticleMolecular and cellular biology1982
Actin from Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1982. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 63 citations in OpenAlex.
- Ultrastructural analysis in yeast reveals a meiosis-specific actin-containing nuclear bundle.Communications biology · 2021Article
- Actin R256 Mono-methylation Is a Conserved Post-translational Modification Involved in Transcription.Cell reports · 2020Article
- Mutant vascular actin is a TAAD misbehaving.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Mutant profilin suppresses mutant actin-dependent mitochondrial phenotype in Saccharomyces cerevisiae.The Journal of biological chemistry · 2011Article
- A potential yeast actin allosteric conduit dependent on hydrophobic core residues val-76 and trp-79.The Journal of biological chemistry · 2010Article
- Meiotic telomere clustering requires actin for its formation and cohesin for its resolution.The Journal of cell biology · 2005Article
- Hierarchy of protein assembly at the vertex ring domain for yeast vacuole docking and fusion.The Journal of cell biology · 2003Article
- Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes.Molecular biology of the cell · 1997Article
- Actin filaments in yeast are unstable in the absence of capping protein or fimbrin.The Journal of cell biology · 1995Article
- Yeast mitochondria contain ATP-sensitive, reversible actin-binding activity.Molecular biology of the cell · 1994Article
- Actin structure and function: roles in mitochondrial organization and morphogenesis in budding yeast and identification of the phalloidin-binding site.Molecular biology of the cell · 1993Article
- SAC1p is an integral membrane protein that influences the cellular requirement for phospholipid transfer protein function and inositol in yeast.The Journal of cell biology · 1993Article
- Molecular genetics of actin function.The Biochemical journal · 1993Review
- Yeast actin filaments display ATP-dependent sliding movement over surfaces coated with rabbit muscle myosin.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Site-directed mutagenesis of the yeast actin gene: a test for actin function in vivo.The EMBO journal · 1991Article
- Spermidine or spermine is essential for the aerobic growth of Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- A polypeptide of 59 kDa is associated with bundles of cytoplasmic filaments in Neurospora crassa.The Biochemical journal · 1990Article
- Lamin A, lamin B, and lamin B receptor analogues in yeast.The Journal of cell biology · 1989Article
- Purification of tropomyosin from Saccharomyces cerevisiae and identification of related proteins in Schizosaccharomyces and Physarum.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Yeast actin-binding proteins: evidence for a role in morphogenesis.The Journal of cell biology · 1988Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Inhibition of DNase I activity has been used as an assay to purify actin from Saccharomyces cerevisiae (yeast actin). The final fraction, obtained after a 300-fold purification, is approximately 97% pure as judged by sodium dodecyl sulfate-gel electrophoresis. Like rabbit skeletal muscle actin, yeast actin has a molecular weight of about 43,000, forms 7-nm-diameter filaments when polymerization is induced by KCl or Mg2+, and can be decorated with a proteolytic fragment of muscle myosin (heavy meromyosin). Although heavy meromyosin ATPase activity is stimulated by rabbit muscle and yeast actins to approximately the same Vmax (2 mmol of Pi per min per mumol of heavy meromyosin), half-maximal activation (Kapp) is obtained with 14 micro M muscle actin, but requires approximately 135 micro M yeast actin. This difference suggests a low affinity of yeast actin for muscle myosin. Yeast and muscle filamentous actin respond similarly to cytochalasin and phalloidin, although the drugs have no effect on S. cerevisiae cell growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.