ArticleThe Journal of cell biology1984
Purification of a high molecular weight actin filament gelation protein from Acanthamoeba that shares antigenic determinants with vertebrate spectrins.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 83 citations in OpenAlex.
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- Computational Tool to Study Perturbations in Muscle Regulation and Its Application to Heart Disease.Biophysical journal · 2019Article
- Analysis of the proteinaceous components of the organic matrix of calcitic sclerites from the soft coral Sinularia sp.PloS one · 2013Article
- Myosin IC generates power over a range of loads via a new tension-sensing mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Structure of the FH2 domain of Daam1: implications for formin regulation of actin assembly.Journal of molecular biology · 2007Article
- Temperature dependence of nucleotide association and kinetic characterization of myo1b.Biochemistry · 2006Article
- Proteolysis of cortactin by calpain regulates membrane protrusion during cell migration.Molecular biology of the cell · 2006Article
- Defective proboscis extension response (DPR), a member of the Ig superfamily required for the gustatory response to salt.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- A subset of dynamic actin rearrangements in Drosophila requires the Arp2/3 complex.The Journal of cell biology · 2002Article
- Profilin binding to poly-L-proline and actin monomers along with ability to catalyze actin nucleotide exchange is required for viability of fission yeast.Molecular biology of the cell · 2001Article
- Caenorhabditis elegans beta-G spectrin is dispensable for establishment of epithelial polarity, but essential for muscular and neuronal function.The Journal of cell biology · 2000Article
- LvsA, a protein related to the mouse beige protein, is required for cytokinesis in Dictyostelium.Molecular biology of the cell · 1999Article
- The interaction of Arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filaments.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Arp2/3 complex from Acanthamoeba binds profilin and cross-links actin filaments.Molecular biology of the cell · 1998Article
- Isoforms of ankyrin-3 that lack the NH2-terminal repeats associate with mouse macrophage lysosomes.The Journal of cell biology · 1997Article
- Sequences, structural models, and cellular localization of the actin-related proteins Arp2 and Arp3 from Acanthamoeba.The Journal of cell biology · 1995Article
- Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose.The Journal of cell biology · 1994Article
- Distinct roles of the Drosophila ninaC kinase and myosin domains revealed by systematic mutagenesis.The Journal of cell biology · 1993Article
- Alpha-spectrin immunoanalog in Acanthamoeba cells.Histochemistry · 1990Article
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Funding
Abstract
I have purified a high molecular weight actin filament gelation protein (GP-260) from Acanthamoeba castellanii, and found by immunological cross-reactivity that it is related to vertebrate spectrins, but not to two other high molecular weight actin-binding proteins, filamin or the microtubule-associated protein, MAP-2. GP-260 was purified by chromatography on DEAE-cellulose, selective precipitation with actin and myosin-II, chromatography on hydroxylapatite in 0.6 M Kl, and selective precipitation at low ionic strength. The yield was 1-2 micrograms/g cells. GP-260 had the same electrophoretic mobility in SDS as the 260,000-mol-wt alpha-chain of spectrin from pig erythrocytes and brain. Electron micrographs of GP-260 shadowed on mica showed slender rod-shaped particles 80-110 nm long. GP-260 raised the low shear apparent viscosity of solutions of Acanthamoeba actin filaments and, at 100 micrograms/ml, formed a gel with a 8 microM actin. Purified antibodies to GP-260 reacted with both 260,000- and 240,000-mol-wt polypeptides in samples of whole ameba proteins separated by gel electrophoresis in SDS, but only the 260,000-mol-wt polypeptide was extracted from the cell with 0.34 M sucrose and purified in this study. These antibodies to GP-260 also reacted with purified spectrin from pig brain and erythrocytes, and antibodies to human erythrocyte spectrin bound to GP-260 and the 240,000-mol-wt polypeptide present in the whole ameba. The antibodies to GP-260 did not bind to chicken gizzard filamin or pig brain MAP-2, but they did react with high molecular weight polypeptides from man, a marsupial, a fish, a clam, a myxomycete, and two other amebas. Fluorescent antibody staining with purified antibodies to GP-260 showed that it is concentrated near the plasma membrane in the ameba.
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