ArticleThe Journal of cell biology1984
Differential control of cytokeratins and vimentin synthesis by cell-cell contact and cell spreading in cultured epithelial cells.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 202 citations in OpenAlex.
- Investigating the effects of essential oils and pure botanical compounds against Eimeria tenella in vitro.Poultry science · 2023Article
- Establishment of a Suspension MDBK Cell Line in Serum-Free Medium for Production of Bovine Alphaherpesvirus-1.Vaccines · 2021Article
- Effect of Weightlessness on the 3D Structure Formation and Physiologic Function of Human Cancer Cells.BioMed research international · 2019Review
- Site-specific glycosylation regulates the form and function of the intermediate filament cytoskeleton.eLife · 2018Article
- Involvement of Rho GAP GRAF1 in maintenance of epithelial phenotype.Cell adhesion & migration · 2017Article
- Cytokeratin 8 in Association with sdLDL and ELISA Development.North American journal of medical sciences · 2015Article
- Intermediate Filaments Play a Pivotal Role in Regulating Cell Architecture and Function.The Journal of biological chemistry · 2015Review
- Hyperoxia induces alveolar epithelial-to-mesenchymal cell transition.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Cell adhesion and shape regulate TGF-beta1-induced epithelial-myofibroblast transition via MRTF-A signaling.PloS one · 2013Article
- Article
- A new permanent cell line derived from the bank vole (Myodes glareolus) as cell culture model for zoonotic viruses.Virology journal · 2011Article
- Undifferentiated carcinoma of the common bile duct with intraductal tumor thrombi: report of a case.Surgery today · 2011Review
- Coordinating changes in cell adhesion and phenotype during EMT-like processes in cancer.F1000 biology reports · 2010Article
- LG4-5 domains of laminin-211 binds alpha-dystroglycan to allow myotube attachment and prevent anoikis.Journal of cellular physiology · 2010Article
- Change in cell shape is required for matrix metalloproteinase-induced epithelial-mesenchymal transition of mammary epithelial cells.Journal of cellular biochemistry · 2008Article
- Epithelial-mesenchymal transition (EMT) of renal tubular cells in canine glomerulonephritis.Virchows Archiv : an international journal of pathology · 2007Article
- Studies on the differentiation pathway and growth characteristics of epithelial culture cells of the human prostate.Prostate cancer and prostatic diseases · 2004Article
- Identification of M-cells in the rabbit tonsil by vimentin immunohistochemistry and in vivo protein transport.Histochemistry and cell biology · 1995Article
- Intermediate filament typing of the human embryonic and fetal notochord.Cell and tissue research · 1995Article
- Adhesion molecules involved in the binding and subsequent engulfment of thymocytes by a rat thymic epithelial cell line.Immunology · 1994Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of cytokeratins and vimentin was investigated in Madin-Darby bovine epithelial cells (MDBK) in culture under conditions of varied cell spreading and cell-cell contact. When extensive cell-cell contact was achieved by seeding cells at high density in monolayer, or in suspension culture in which multicellular aggregates formed, the cells synthesized high levels of cytokeratins and low levels of vimentin. In contrast, in sparse monolayer and suspension cultures where cell-cell contact was minimal, the cells synthesized very low levels of cytokeratins. The level of vimentin synthesis was high in sparse monolayer culture and was low in both sparse and dense suspension cultures. The ratio of cytokeratin to vimentin synthesis was not affected during the cell cycle, or when cell growth was inhibited by ara C and in serum-starvation-stimulation experiments. The variations in the synthesis of cytokeratins and vimentin under the various culture conditions were also reflected at the level of mRNA activity in a cell-free in vitro translation system and as determined by RNA blot hybridization with cDNA to vimentin and cytokeratins. The results suggest that control of cytokeratin synthesis involves cell-cell contact, characteristic of epithelia in vivo, while vimentin synthesis responds to alterations in cell spreading.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.