ArticleThe Histochemical journal1984
Optimal techniques for the immunocytochemical demonstration of beta-thromboglobulin, platelet factor 4, and fibrinogen in the alpha granules of unstimulated platelets.
Article in The Histochemical journal, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 51 citations in OpenAlex.
- 3D ultrastructural analysis of α-granule, dense granule, mitochondria, and canalicular system arrangement in resting human platelets.Research and practice in thrombosis and haemostasis · 2020Article
- SNARE-dependent membrane fusion initiates α-granule matrix decondensation in mouse platelets.Blood advances · 2018Article
- The cellular basis of platelet secretion: Emerging structure/function relationships.Platelets · 2017Review
- The end is just the beginning: megakaryocyte apoptosis and platelet release.International journal of hematology · 2001Review
- Role for Bcl-xL as an inhibitor of cytosolic cytochrome C accumulation in DNA damage-induced apoptosis.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- A functional integrin ligand on the surface of platelets: intercellular adhesion molecule-2.The Journal of clinical investigation · 1994Article
- The postmortem activation status of platelets.International journal of legal medicine · 1994Article
- In vivo defibrination results in markedly decreased amounts of fibrinogen in rat megakaryocytes and platelets.The American journal of pathology · 1990Article
- Immunoelectron-microscopic studies of human platelet thrombospondin, von Willebrand factor, and fibrinogen redistribution during clot formation.The Histochemical journal · 1990Article
- Thrombospondin expression in traumatized skeletal muscle. Correlation of appearance with post-trauma regeneration.Cell and tissue research · 1990Article
- Local abnormalities of coagulation and fibrinolytic pathways that promote alveolar fibrin deposition in the lungs of baboons with diffuse alveolar damage.The Journal of clinical investigation · 1989Article
- Electron microscopic and cytochemical studies of the thrombocytes of the tortoise (Geoclemys reevesii).Journal of anatomy · 1987Article
- Regulation of the activity of platelet-bound C3 convertase of the alternative pathway of complement by platelet factor H.Proceedings of the National Academy of Sciences of the United States of America · 1987Article
- Immunoglobulin G is a platelet alpha granule-secreted protein.The Journal of clinical investigation · 1985Article
- A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation.The Journal of cell biology · 1985Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The distribution of beta-thromboglobulin, platelet factor 4, and fibrinogen in unstimulated platelets was investigated by several immunocytochemical techniques. All three substances were found to be localized in the majority of platelet alpha granules either by immunoperoxidase methods on saponin-treated platelets or by colloidal gold immunoconjugates on frozen thin sections. The optimal conditions for preparing and fixing platelets for immunocytochemistry were also determined. Platelets obtained from blood dripped directly into fixative or anticoagulated blood were compared systematically with respect to shape. Temperature was found to be the most important variable. Immediately fixed platelets were generally disc-shaped, regardless of the temperature of the fixative. Reducing the temperature of blood (stored with anticoagulant) before fixation resulted in more swollen and fewer disc-shaped platelets. However, if the blood was mixed with an anticoagulant and maintained at 37 degrees C for 1 h before fixation, the same number of disc-shaped platelets were present as in samples from blood fixed immediately. The intracellular localization of beta-thromboglobulin, platelet factor 4, and fibrinogen was consistent regardless of platelet preparatory procedure, but several technical problems were encountered with respect to plasma membrane labelling when control experiments were analysed. Immediately fixed, non-permeabilized platelet plasma membranes were always labelled, no matter which control substances or immunoperoxidase markers were used. However, when platelets were washed by centrifugation, the plasma membranes were negative. Exposure to saponin markedly diminished labelling of the plasma membranes. Optimal techniques for the immunocytochemical demonstration of these alpha granule proteins in platelets are presented in this report.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.