ArticleThe Histochemical journal1984
Histochemical identification of side chain substituted O-acylated sialic acids: the PAT-KOH-Bh-PAS and the PAPT-KOH-Bh-PAS procedures.
Article in The Histochemical journal, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Reduction of sialic acid O-acetylation in human colonic mucins in the adenoma-carcinoma sequence.Glycoconjugate journal · 1999Article
- Mucin staining.Journal of clinical pathology · 1996Article
- Histochemical analysis of glycoproteins in the unicellar glands in the epidermis of an Indian freshwater fish Mastacembelus pancalus (Hamilton).The Histochemical journal · 1994Article
- Distribution of O-acetylated sialomucin in the normal and diseased gastrointestinal tract shown by a new monoclonal antibody.Journal of clinical pathology · 1993Article
- Patterns of intestinal metaplasia in gastric biopsies. A comparison of different histochemical classifications.The Histochemical journal · 1991Article
- Histochemical classification based upon reaction types and its application to carbohydrate histochemistry.The Histochemical journal · 1990Review
- Histochemical studies of the colonic epithelial glycoproteins of the normal rabbit.The Histochemical journal · 1988Article
- A new method for the histochemical demonstration of O-acyl sugars in human colonic epithelial glycoproteins.The Histochemical journal · 1988Article
- Histochemical procedures for the simultaneous visualization of neutral sugars and either sialic acid and its O-acyl variants or O-sulphate ester. II. Methods based upon the periodic acid-phenylhydrazine-Schiff reaction.The Histochemical journal · 1987Article
- Histochemical procedures for the simultaneous visualization of neutral sugars and either sialic acid and its side chain O-acyl variants or O-sulphate ester. I. Methods based upon the selective periodate oxidation of sialic acids.The Histochemical journal · 1987Article
- Can 'mild' periodate oxidation be used for the specific histochemical identification of sialic acid residues?The Histochemical journal · 1986Article
- O-acylated sialic acid variants in mucinous tumours of the ovary.Virchows Archiv. A, Pathological anatomy and histopathology · 1986Article
- Chemical and histochemical studies of normal and diseased human gastrointestinal tract. V. A differential diagnostic method for the histochemical classification of glycoproteins.The Histochemical journal · 1985Article
- Chemical and histochemical studies of normal and diseased human gastrointestinal tract. III. Changes in the histochemical and chemical properties of the epithelial glycoproteins in the mucosa close to colonic tumours.The Histochemical journal · 1985Article
- Chemical and histochemical studies of normal and diseased gastrointestinal tract. IV. A comparison of histochemical and chemical methods for the estimation of side chain O-acylated sialic acids.The Histochemical journal · 1985Article
- Histochemical procedures for the simultaneous visualization of sialic acid, its side chain O-acyl variants and O-sulphate ester.The Histochemical journal · 1985Article
- Histochemical studies of the mechanism of the periodic acid-phenylhydrazine-Schiff (PAPS) procedure.The Histochemical journal · 1984Article
- Methods for the identification of side chain O-acyl substituted sialic acids and for the simultaneous visualization of sialic acid, its side chain O-acyl variants and O-sulphate ester.The Histochemical journalArticle
- A new histochemical method for the selective periodate oxidation of total tissue sialic acids.The Histochemical journalArticle
- Histochemical studies of epithelial cell glycoproteins in normal rat colon.The Histochemical journalArticle
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Abstract
Two new methods, based on the original periodic acid-Thionin Schiff-saponification-periodic acid-Basic Fuchsin Schiff (PAT-KOH-PAS) technique of Culling et al. (1976), have been devised for the histochemical identification of side-chain O-acylated sialic acids. In the first of these, the periodic acid-Thionin Schiff-saponification-borohydride reduction-periodic acid-Basic Fuchsin Schiff (PAT-KOH-Bh-PAS) procedure, the specificity of the original PAT-KOH-PAS technique was improved by: (a) extending, when necessary, the initial period of periodate oxidation, (b) increasing the period of exposure to Thionin Schiff reagent from 30 min to 4 h, (c) using a Thionin Schiff reagent prepared by a different method, (d) interposing a borohydride reduction step between the saponification and PAS steps and, (e) extending the period of oxidation in the final PAS step from 10 to 30 min. In the second procedure, the periodic acid-phenylhydrazine-Thionin Schiff-borohydride reduction-periodic acid-Basic Fuchsin Schiff (PAPT-KOH-Bh-PAS), based on the periodic acid-phenylhydrazine-Schiff (PAPS) technique of Spicer (1961), blue Thionin Schiff staining was confined to sialic acid residues with oxidizable side chain vicinal diols by interposing a treatment with 0.5% (w/v) aqueous phenylhydrazine hydrochloride for 2 h at room temperature between the initial periodic acid oxidation and the Thionin Schiff steps of the PAT-KOH-Bh-PAS procedure. These procedures are discussed within the general framework of the methods available for the histochemical identification of side-chain O-acylated sialic acids.
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