ArticleThe Journal of cell biology1984
Monomer-polymer equilibria in the axon: direct measurement of tubulin and actin as polymer and monomer in axoplasm.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 115 citations in OpenAlex.
- A Quantitative Systems Pharmacology Model That Describes Neurofilament Light Dynamics During Alzheimer's Disease Progression.CPT: pharmacometrics & systems pharmacology · 2025Article
- The Axonal Actin Filament Cytoskeleton: Structure, Function, and Relevance to Injury and Degeneration.Molecular neurobiology · 2024Review
- Functional Genomics of Axons and Synapses to Understand Neurodegenerative Diseases.Frontiers in cellular neuroscience · 2021Review
- Neurofilaments in disease: what do we know?Current opinion in neurobiology · 2020Review
- Dynamical decoration of stabilized-microtubules by Tau-proteins.Scientific reports · 2019Article
- Processive flow by biased polymerization mediates the slow axonal transport of actin.The Journal of cell biology · 2019Article
- HIV Glycoprotein Gp120 Impairs Fast Axonal Transport by Activating Tak1 Signaling Pathways.ASN neuro · 2016Article
- Bidirectional actin transport is influenced by microtubule and actin stability.Cellular and molecular life sciences : CMLS · 2015Article
- Comparison of [corrected] actin- and glass-supported phospholipid bilayer diffusion coefficients.Biophysical journal · 2015Article
- Analysis of microtubules in isolated axoplasm from the squid giant axon.Methods in cell biology · 2013Article
- Quantitative measurements and modeling of cargo-motor interactions during fast transport in the living axon.Physical biology · 2012Article
- Mitotic spindle form and function.Genetics · 2012Article
- Scapinin-induced inhibition of axon elongation is attenuated by phosphorylation and translocation to the cytoplasm.The Journal of biological chemistry · 2011Article
- Tension management in the kinetochore.Current biology : CB · 2010Review
- A diffusion-based neurite length-sensing mechanism involved in neuronal symmetry breaking.Molecular systems biology · 2010Article
- The amino terminus of tau inhibits kinesin-dependent axonal transport: implications for filament toxicity.Journal of neuroscience research · 2009Article
- In vivo assay of presynaptic microtubule cytoskeleton dynamics in Drosophila.Journal of neuroscience methods · 2007Article
- Tubulin and neurofilament proteins are transported differently in axons of chicken motoneurons.Cellular and molecular neurobiology · 2000Article
- A role for cyclin-dependent kinase(s) in the modulation of fast anterograde axonal transport: effects defined by olomoucine and the APC tumor suppressor protein.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- A new perspective on microtubules and axon growth.The Journal of cell biology · 1993Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The monomer-polymer equilibria for tubulin and actin were analyzed for the cytoskeleton of the squid giant axon. Two methods were evaluated for measuring the concentrations of monomer, soluble (equilibrium) polymer, and stable polymer in extruded axoplasm. One method, the Kinetic Equilibration Paradigm ( KEP ), employs the basic principles of diffusion to distinguish freely diffusible monomer from proteins that are present in the form of polymer. The other method is pharmacological and employs either taxol or phalloidin to stabilize the microtubules and microfilaments, respectively. The results of the two methods agree and demonstrate that 22-36% of the tubulin and 41-47% of the actin are monomeric. The in vivo concentration of monomeric actin and tubulin were two to three times higher than the concentration required to polymerize these proteins in vitro, suggesting that assembly of these proteins is regulated by additional mechanisms in the axon. A significant fraction of the polymerized actin and tubulin in the axoplasm was stable microtubules and microfilaments, which suggests that the dissociation reaction is blocked at both ends of these polymers. These results are discussed in relationship to the axonal transport of the cytoskeleton and with regard to the ability of axons to change their shape in response to environmental stimuli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.