ArticleThe Journal of cell biology1984
Microspectrofluorometry by digital image processing: measurement of cytoplasmic pH.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 133 citations in OpenAlex.
- Article
- PiQSARS: A pipeline for quantitative and statistical analyses of ratiometric fluorescent biosensors.MethodsX · 2020Article
- Correlative Light Electron Microscopy: Connecting Synaptic Structure and Function.Frontiers in synaptic neuroscience · 2016Review
- Anima: modular workflow system for comprehensive image data analysis.Frontiers in bioengineering and biotechnology · 2014Article
- Low pH immobilizes and kills human leukocytes and prevents transmission of cell-associated HIV in a mouse model.BMC infectious diseases · 2005Article
- Molecular determinants of synapsin targeting to presynaptic terminals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Fluorescence lifetime-resolved pH imaging of living cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2003Article
- Myosin II transport, organization, and phosphorylation: evidence for cortical flow/solation-contraction coupling during cytokinesis and cell locomotion.Molecular biology of the cell · 1996Article
- Gradients in the concentration and assembly of myosin II in living fibroblasts during locomotion and fiber transport.Molecular biology of the cell · 1993Article
- Fluorescence anisotropy imaging microscopy maps calmodulin binding during cellular contraction and locomotion.The Journal of cell biology · 1993Article
- Mutagenesis of the putative fusion domain of the Semliki Forest virus spike protein.Journal of virology · 1991Article
- Biosynthesis, maturation, and acid activation of the Semliki Forest virus fusion protein.Journal of virology · 1990Article
- Video microscopy of intracellular pH in primary cultures of rabbit proximal and early distal tubules.Pflugers Archiv : European journal of physiology · 1990Article
- The exit of Trypanosoma cruzi from the phagosome is inhibited by raising the pH of acidic compartments.The Journal of experimental medicine · 1990Article
- A possible role for Na+,K+-ATPase in regulating ATP-dependent endosome acidification.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- The dynamic distribution of fluorescent analogues of actin and myosin in protrusions at the leading edge of migrating Swiss 3T3 fibroblasts.The Journal of cell biology · 1988Article
- Aldolase exists in both the fluid and solid phases of cytoplasm.The Journal of cell biology · 1988Article
- Acidification of morphologically distinct endosomes in mutant and wild-type Chinese hamster ovary cells.The Journal of cell biology · 1987Article
- Five-parameter fluorescence imaging: wound healing of living Swiss 3T3 cells.The Journal of cell biology · 1987Article
- Fluorescence ratio imaging microscopy: temporal and spatial measurements of cytoplasmic pH.The Journal of cell biology · 1987Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An interface of our microspectrofluorometer with an image processing system performs microspectrofluorometric measurements in living cells by digital image processing. Fluorescence spectroscopic parameters can be measured by digital image processing directly from microscopic images of cells, and are automatically normalized for pathlength and accessible volume. Thus, an accurate cytoplasmic "map" of various spectroscopic parameters can be produced. The resting cytoplasmic pH of fibroblasts (3T3 cells) has been determined by measuring the ratio of fluorescein fluorescence exited by two successive wavelengths (489 and 452 nm). Fluorescein-labeled dextran microinjected into the cells is used as a pH indicator, since it is trapped in the cytoplasm but is excluded from the nucleus and other organelles. The average cytoplasmic pH is 6.83 (+/- 0.38). However, cytoplasmic pH exhibits a nonunimodal distribution, the lower mean pH being 6.74 (+/- 0.23). When 3T3 cells pinocytose medium containing fluorescein dextran, pinosomes peripheral to the nucleus exhibit a lower pH than those closer to the ruffling edge of the cell. The present image processing system is analyzed for linearity of detection, light scattering artifacts, signal to noise ratio, standard curves, and spatial resolution. The results obtained from digital image analysis are shown to be comparable to the results from standard microspectrofluorometry. We also discuss several other applications of this ratio imaging technique in cell biology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.