ArticleProceedings of the National Academy of Sciences of the United States of America1982
Molecular cloning of mRNA sequences for cardiac alpha- and beta-form myosin heavy chains: expression in ventricles of normal, hypothyroid, and thyrotoxic rabbits.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1982. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 131 citations in OpenAlex.
- Functional consequences of the human cardiac troponin I hypertrophic cardiomyopathy mutation R145G in transgenic mice.The Journal of biological chemistry · 2008Article
- The survival of embryonic cardiomyocytes transplanted into damaged host rat myocardium.Journal of muscle research and cell motility · 1997Article
- Overexpression of insulin-like growth factor-1 in the heart is coupled with myocyte proliferation in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- An in vivo analysis of transcriptional elements in the mouse alpha-myosin heavy chain gene promoter.Transgenic research · 1995Article
- Identification of alpha-cardiac myosin heavy chain mRNA and protein in extraocular muscle of the adult rabbit.Journal of muscle research and cell motility · 1994Article
- Dietary protein levels affect growth and protein metabolism in trunk muscle of cod, Gadus morhua.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 1992Article
- Structural and phylogenetic analysis of the chicken ventricular myosin heavy chain rod.Journal of molecular evolution · 1991Article
- Role of thyroid hormones in apolipoprotein A-I gene expression in rat liver.The Journal of clinical investigation · 1990Article
- Negative regulation of the thyroid-stimulating hormone alpha gene by thyroid hormone: receptor interaction adjacent to the TATA box.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Characterization of human cardiac myosin heavy chain genes.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Regulation of myosin heavy-chain gene expression during skeletal-muscle hypertrophy.The Biochemical journal · 1989Article
- RNA transcription in myocardial-cell nuclei during postnatal development. A study establishing an assay system for transcription in vitro.The Biochemical journal · 1988Article
- Molecular cloning and characterization of human cardiac alpha- and beta-form myosin heavy chain complementary DNA clones. Regulation of expression during development and pressure overload in human atrium.The Journal of clinical investigation · 1988Article
- Regulation of gene expression by thyroid hormone.The Journal of clinical investigation · 1988Review
- Visualization of cardiac ventricular myosin heavy chain homodimers and heterodimers by monoclonal antibody epitope mapping.The Journal of cell biology · 1987Article
- Human cardiac myosin heavy chain genes and their linkage in the genome.Nucleic acids research · 1987Article
- A combination of RNase H and S1 nuclease circumvents an artefact inherent to conventional S1 analysis of RNA splicing.Nucleic acids research · 1987Article
- Myosin heavy chain messenger RNA and protein isoform transitions during cardiac hypertrophy. Interaction between hemodynamic and thyroid hormone-induced signals.The Journal of clinical investigation · 1987Article
- Influence of V1 and V3 isomyosins on the mechanical behaviour of rat papillary muscle as studied by pseudo-random binary noise modulated length perturbations.Journal of muscle research and cell motility · 1986Article
- Myosin types and fiber types in cardiac muscle. III. Nodal conduction tissue.The Journal of cell biology · 1986Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
We have isolated cDNA clones from thyrotoxic (pMHC alpha) and normal (pMHC beta) adult rabbit hearts. Restriction map analysis and DNA sequence analyses show that, although there is strong homology between overlapping regions of the two clones, they are distinctly different. The two clones exhibited 78-83% homology between the derived amino acid sequences and those determined by direct amino acid sequence analysis of rabbit fast skeletal muscle myosin heavy chains. The clones specify a segment of the myosin heavy chain corresponding to subfragment 2 and the COOH-terminal portions of subfragment 1. Nuclease S1 mapping was used to compare transcription of the two clones with expression of the alpha and beta forms of myosin heavy chains in the ventricles of thyrotoxic, hypothyroid (propylthiouracil-treated), and normal rabbits. Thyrotoxic ventricles contained only pMHC alpha transcripts whereas hypothyroid ventricles contained exclusively pMHC beta transcripts. These data correlate well with the presence of alpha- and beta-form myosin heavy chains. In the normal young adult rabbit, pMHC beta transcripts predominate, agreeing with the known beta form/alpha form ratio of 4:1. We therefore conclude that pMHC alpha and pMHC beta contain sequences of the alpha- and beta-form myosin heavy chain genes, respectively.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.