ArticleThe Journal of cell biology1982
Evidence that myosin does not contribute to force production in chromosome movement.
Article in The Journal of cell biology, 1982. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 123 citations in OpenAlex.
- Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.Frontiers in cell and developmental biology · 2024Article
- Cleavage-furrow formation without F-actin inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cell-intrinsic and -extrinsic mechanisms promote cell-type-specific cytokinetic diversity.eLife · 2018Article
- The biology of the germ line in echinoderms.Molecular reproduction and development · 2014Review
- And the dead shall rise: actin and myosin return to the spindle.Developmental cell · 2011Review
- Actomyosin-dependent cortical dynamics contributes to the prophase force-balance in the early Drosophila embryo.PloS one · 2011Article
- Inhibitors of myosin, but not actin, alter transport through Tradescantia plasmodesmata.Protoplasma · 2011Article
- The perpetual movements of anaphase.Cellular and molecular life sciences : CMLS · 2010Review
- Myosin-10 and actin filaments are essential for mitotic spindle function.The Journal of cell biology · 2008Article
- Article
- Calcium-responsive contractility during fertilization in sea urchin eggs.Developmental dynamics : an official publication of the American Association of Anatomists · 2006Article
- Calcium at fertilization and in early development.Physiological reviews · 2006Review
- The Rho GTP exchange factor Lfc promotes spindle assembly in early mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Cytochalasin D and latrunculin affect chromosome behaviour during meiosis in crane-fly spermatocytes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1998Article
- Differential expression and functions of cortical myosin IIA and IIB isotypes during meiotic maturation, fertilization, and mitosis in mouse oocytes and embryos.Molecular biology of the cell · 1998Article
- Myosin heavy chain phosphorylation sites regulate myosin localization during cytokinesis in live cells.Molecular biology of the cell · 1997Article
- Myosin II transport, organization, and phosphorylation: evidence for cortical flow/solation-contraction coupling during cytokinesis and cell locomotion.Molecular biology of the cell · 1996Article
- A novel member of the rho family of small GTP-binding proteins is specifically required for cytokinesis.The Journal of cell biology · 1996Article
- Characterization of the nonmuscle myosin heavy chain IIB promoter: regulation by E2F.Gene expression · 1996Article
- A fluorescent protein biosensor of myosin II regulatory light chain phosphorylation reports a gradient of phosphorylated myosin II in migrating cells.Molecular biology of the cell · 1995Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Antibody against cytoplasmic myosin, when microinjected into actively dividing cells, provides a physiological test for the role of actin and myosin in chromosome movement. Anti-Asterias egg myosin, characterized by Mabuchi and Okuno (1977, J. Cell Biol., 74:251), completely and specifically inhibits the actin activated Mg++ -ATPase of myosin in vitro and, when microinjected, inhibits cytokinesis in vivo. Here, we demonstrate that microinjected antibody has no observable effect on the rate or extent of anaphase chromosome movements. Neither central spindle elongation nor chromosomal fiber shortening is affected by doses up to eightfold higher than those require to uniformly inhibit cytokinesis in all injected cells. We calculate that such doses are sufficient to completely inhibit myosin ATPase activity in these cells. Cells injected with buffer alone, with myosin-absorbed antibody, or with nonimmune gamma-globulin, proceed normally through both mitosis and cytokinesis. Control gamma-globulin, labeled with fluorescein, diffuses to homogeneity throughout the cytoplasm in 2-4 min and remains uniformly distributed. Antibody is not excluded from the spindle region. Prometaphase chromosome movements, fertilization, pronuclear migration, and pronuclear fusion are also unaffected by microinjected antimyosin. These experiments demonstrate that antimyosin blocks the actomyosin interaction thought to be responsible for force production in cytokinesis but has no effect on mitotic or meiotic chromosome motion. They provide direct physiological evidence that myosin is not involved in force production for chromosome movement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.