ArticleThe Journal of cell biology1981
A nucleolar skeleton of protein filaments demonstrated in amplified nucleoli of Xenopus laevis.
Article in The Journal of cell biology, 1981. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 80 citations in OpenAlex.
- Protein Aggregation and Disaggregation in Cells and Development.Journal of molecular biology · 2021Review
- Article
- Amyloids assemble as part of recognizable structures during oogenesis in Xenopus.Biology open · 2016Article
- Evolutionary history of the mammalian synaptonemal complex.Chromosoma · 2016Review
- NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin.Molecular biology of the cell · 2004Article
- A novel karyoskeletal protein: characterization of protein NO145, the major component of nucleolar cortical skeleton in Xenopus oocytes.Molecular biology of the cell · 2001Article
- Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs.The EMBO journal · 1998Article
- Molecular characterization of a novel, widespread nuclear protein that colocalizes with spliceosome components.Molecular biology of the cell · 1998Article
- Multiparameter microscopic analysis of nucleolar structure and ribosomal gene transcription.Histochemistry and cell biology · 1996Review
- Structural changes in oocyte nucleoli of Xenopus laevis during oogenesis and meiotic maturation.Chromosoma · 1996Article
- Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential.Molecular and cellular biology · 1995Article
- NAP57, a mammalian nucleolar protein with a putative homolog in yeast and bacteria.The Journal of cell biology · 1994Article
- The nucleolus.Anatomy and embryology · 1993Review
- Intranuclear filaments containing a nuclear pore complex protein.The Journal of cell biology · 1993Article
- Visualization of focal sites of transcription within human nuclei.The EMBO journal · 1993Article
- Binding of sequences from the 5'- and 3'-nontranscribed spacers of the rat rDNA locus to the nucleolar matrix.Chromosoma · 1993Article
- A nuclear localization signal binding protein in the nucleolus.The Journal of cell biology · 1990Article
- Inhibition of nucleolar reformation after microinjection of antibodies to RNA polymerase I into mitotic cells.The Journal of cell biology · 1987Article
- A constitutive nucleolar protein identified as a member of the nucleoplasmin family.The EMBO journal · 1987Article
- Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The amplified, extrachromosomal nucleoli of Xenopus oocytes contain a meshwork of approximately 4-nm-thick filaments, which are densely coiled into higher-order fibrils of diameter 30-40 nm and are resistant to treatment with high- and low-salt concentrations, nucleases (DNase I, pancreatic RNase, micrococcal nuclease), sulfhydryl agents, and various nonionic detergents. This filamentous "skeleton" has been prepared from manually isolated nuclear contents and nucleoli as well as from nucleoli isolated by fluorescence-activated particle sorting. The nucleolar skeletons are observed in light and electron microscopy and are characterized by ravels of filaments that are especially densely packed in the nucleolar cortex. DNA as well as RNA are not constituents of this structure, and precursors to ribosomal RNAs are completely removed from the extraction-resistant filaments by treatment with high-salt buffer or RNase. Fractions of isolated nucleolar skeletons show specific enrichment of an acidic major protein of 145,000 mol wt and an apparent pI value of approximately 6.15, accompanied in some preparations by various amounts of minor proteins. The demonstration of this skeletal structure in "free" extrachromosomal nucleoli excludes the problem of contaminations by nonnucleolar material such as perinucleolar heterochromatin normally encountered in studies of nucleoli from somatic cells. It is suggested that this insoluble protein filament complex forms a skeleton specific to the nucleolus proper that is different from other extraction-resistant components of the nucleus such as matrix and lamina and is involved in the spatial organization of the nucleolar chromatin and its transcriptional products.
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