ArticleThe Journal of cell biology1984
Taxol-induced bundling of brain-derived microtubules.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 55 citations in OpenAlex.
- Paclitaxel compromises nuclear integrity in interphase through SUN2-mediated cytoskeletal coupling.Journal of cell science · 2026Article
- Pocket similarity identifies selective estrogen receptor modulators as microtubule modulators at the taxane site.Nature communications · 2019Article
- Mitochondria-enriched protrusions are associated with brain and intestinal stem cells inCommunications biology · 2019Article
- Beyond taxol: microtubule-based treatment of disease and injury of the nervous system.Brain : a journal of neurology · 2013Review
- Peloruside A inhibits microtubule dynamics in a breast cancer cell line MCF7.Investigational new drugs · 2011Article
- Induction of paclitaxel resistance by the Kaposi's sarcoma-associated herpesvirus latent protein LANA2.Journal of virology · 2008Article
- Taxol crystals can masquerade as stabilized microtubules.PloS one · 2008Article
- Taxol-induced apoptosis depends on MAP kinase pathways (ERK and p38) and is independent of p53.Oncogene · 2001Article
- Ultrastructural neuropathologic effects of Taxol on neurons of the freshwater snail Lymnaea stagnalis.Journal of neuro-oncology · 1995Article
- Biochemical and immunochemical analysis of rat brain dynamin interaction with microtubules and organelles in vivo and in vitro.The Journal of cell biology · 1990Article
- Effects of two microtubule-depolymerizing drugs, vincristine and vinblastine, on porphyrin production by primary neural tissue cultures.Neurochemical research · 1990Article
- Kinetics of the spontaneous organization of microtubules in solution.European biophysics journal : EBJ · 1990Article
- Stable complexes of axoplasmic vesicles and microtubules: protein composition and ATPase activity.The Journal of cell biology · 1986Article
- Microtubules and microtubule-associated proteins from the nematode Caenorhabditis elegans: periodic cross-links connect microtubules in vitro.The Journal of cell biology · 1986Article
- Monoclonal antibody to intermediate filament antigen cross-reacts with higher plant cells.The Journal of cell biology · 1985Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Taxol has two obvious effects in cells. It stabilizes microtubules and it induces microtubule bundling. We have duplicated the microtubule-bundling effect of taxol in vitro and report preliminary characterization of this bundling using electron microscopy, sedimentation, and electrophoretic analyses. Taxol-bundled microtubules from rat brain crude extracts were seen as massive bundles by electron microscopy. Bundled microtubules sedimented through sucrose five times faster than control microtubules. Electrophoretic analysis of control and taxol-bundled microtubules pelleted through sucrose revealed no striking differences between the two samples except for a protein doublet of approximately 100,000 daltons. Taxol-induced microtubule bundling was not produced by using pure tubulin or recycled microtubule protein; this suggested that taxol-induced microtubule bundling was mediated by a factor present in rat brain crude extracts. Taxol cross-linked rat brain crude extract microtubules were entirely labile to ATP in the millimolar range. This ATP-dependent relaxation was also demonstrated in a more purified system, using taxol-bundled microtubules pelleted through sucrose and gently resuspended. Although the bundling factor did not recycle with microtubule protein, it was apparently retained on isolated taxol-stabilized microtubules. The bundling factor was salt extracted from taxol-stabilized microtubules and its retained activity was demonstrated in an add-back experiment with assembled phosphocellulose-purified tubulin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.