ArticleThe Journal of cell biology1984
Taxol-induced rose microtubule polymerization in vitro and its inhibition by colchicine.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 69 citations in OpenAlex.
- Microtubule drugs: action, selectivity, and resistance across the kingdoms of life.Protoplasma · 2014Review
- Rationalization of paclitaxel insensitivity of yeast β-tubulin and human βIII-tubulin isotype using principal component analysis.BMC research notes · 2012Article
- Stat3 regulates microtubules by antagonizing the depolymerization activity of stathmin.The Journal of cell biology · 2006Article
- Molecular markers and cell cycle inhibitors show the importance of cell cycle progression in nematode-induced galls and syncytia.The Plant cell · 1999Article
- Effect of microtubule stabilization on the freezing tolerance of mesophyll cells of spinach.Plant physiology · 1991Article
- The use of antimicrotubule herbicides for the production of doubled haploid plants from anther-derived maize callus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1991Article
- Effects of microtubule agents on the spatial and electrical properties of the plasma membrane inChara corallina.Planta · 1990Article
- Extragenic suppression and synthetic lethality among Chlamydomonas reinhardtii mutants resistant to anti-microtubule drugs.Genetics · 1989Article
- Microtubule-binding proteins from carrot : I. Initial characterization and microtubule bundling.Planta · 1989Article
- Nuclear fusion-defective phenocopies in Chlamydomonas reinhardtii: mating-type functions for meiosis can act through the cytoplasm.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Oryzalin, a dinitroaniline herbicide, binds to plant tubulin and inhibits microtubule polymerization in vitro.Planta · 1987Article
- Altered expression of a heat shock protein in the mammalian nervous system in the presence of agents which affect microtubule stability.Neurochemical research · 1987Article
- Article
- Physiological and ultrastructural analysis of elongating mitotic spindles reactivated in vitro.The Journal of cell biology · 1986Article
- Identification of multiple tubulins in taxol microtubules purified from carrot suspension cells.The EMBO journal · 1985Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tubulin was isolated from cultured cells of rose (Rosa, sp.cv. Paul's scarlet) by DEAE-Sephadex A50 chromatography, and the taxol-induced polymerization of microtubules in vitro was characterized at 24 degrees C by turbidity development, sedimentation analysis, and electron microscopy. Numerous, short microtubules were formed in the presence of taxol, and maximum levels of turbidity and polymer yield were obtained at approximately 2:1 molar ratios of taxol to tubulin. The critical concentration of rose tubulin for polymerization in saturating taxol was estimated to be 0.21 mg/ml. Colchicine inhibited the taxol-induced polymerization of tubulin as shown by sedimentation assays; however, much higher concentrations of colchicine were required for the inhibition of taxol-induced rose tubulin assembly than for inhibition of taxol-induced mammalian brain tubulin assembly. On the basis of the relative sensitivity of rose tubulin assembly to taxol and its insensitivity to colchicine, we propose that the taxol-binding site(s) on plant and animal tubulins have been more conserved over evolution than the colchicine-binding site(s).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.