ArticleThe Journal of cell biology1983
Isolation of Chinese hamster ovary cell mutants requiring the continuous presence of taxol for cell division.
Article in The Journal of cell biology, 1983. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 72 citations in OpenAlex.
- Metronomic chemotherapy in progressive pediatric malignancies: old drugs in new package.Indian journal of pediatrics · 2012Review
- Random mutagenesis of β-tubulin defines a set of dispersed mutations that confer paclitaxel resistance.Pharmaceutical research · 2012Article
- New insights into mechanisms of resistance to microtubule inhibitors.Biochimica et biophysica acta · 2011Review
- Mitotic centromere-associated kinesin (MCAK) mediates paclitaxel resistance.The Journal of biological chemistry · 2011Article
- Paclitaxel-dependent cell lines reveal a novel drug activity.Molecular cancer therapeutics · 2010Article
- Inhibition of cell migration and cell division correlates with distinct effects of microtubule inhibiting drugs.The Journal of biological chemistry · 2010Article
- Metronomic chemotherapy: new rationale for new directions.Nature reviews. Clinical oncology · 2010Review
- Response to 'Intermittent androgen blockade should be regarded as standard therapy in prostate cancer'.Nature clinical practice. Oncology · 2009Article
- A ubiquitous beta-tubulin disrupts microtubule assembly and inhibits cell proliferation.Molecular biology of the cell · 2004Article
- Stable expression of heterologous microtubule-associated proteins (MAPs) in Chinese hamster ovary cells: evidence for differing roles of MAPs in microtubule organization.The Journal of cell biology · 1994Article
- Comparative in vitro cytotoxicity of taxol and Taxotere against cisplatin-sensitive and -resistant human ovarian carcinoma cell lines.Cancer chemotherapy and pharmacology · 1992Article
- Interzone microtubule behavior in late anaphase and telophase spindles.The Journal of cell biology · 1987Article
- Taxol-dependent mutants of Chinese hamster ovary cells with alterations in alpha- and beta-tubulin.The Journal of cell biology · 1986Article
- Giant axonal neuropathy: a conditional mutation affecting cytoskeletal organization.The Journal of cell biology · 1985Article
- Genetics of microtubule systems.The Journal of cell biology · 1984Review
- Mutations in alpha- and beta-tubulin affect spindle formation in Chinese hamster ovary cells.The Journal of cell biology · 1983Article
- Taxol-requiring mutant of Chinese hamster ovary cells with impaired mitotic spindle assembly.The Journal of cell biology · 1983Article
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Authors and funding
1 author.
Funding
Abstract
Chinese hamster ovary (CHO) cell mutants resistant to the cytotoxic effects of taxol and requiring the drug for normal growth were isolated in a single step. One of these mutant cell lines, Tax-18, fails to divide in the absence of taxol; instead, the cells become larger, rounder, flatter, and multinucleated. Analysis by flow cytometry indicates that during taxol deprivation there is an accumulation of cells in G2 + M phase but that the cells are able to leak through the block in the absence of cell division and further increase their DNA content beyond the tetraploid amount. This interpretation is confirmed by karyotype analysis and by time-lapse studies that show cells rounded for mitosis two to five times longer than in wild-type cultures or in Tax-18 cultures grown in taxol. The cells finally attempt to undergo cytokinesis, fail, and spread out again, but as larger cells than before. Tax-18 has a normal growth rate and morphology when grown in taxol even at concentrations three to five times below the selecting concentration of the drug. The cells, however, have increased sensitivity to microtubule-disrupting drugs such as colcemid, griseofulvin, and D2O. The mutation for taxol auxotrophy behaves recessively in somatic cell hybridization experiments, and the phenotypic reversion rate is approximately 10(-5) in a nonmutagenized population. Both alpha- and beta-tubulin are present in apparently normal amounts and with normal electrophoretic mobilities on two-dimensional gels. The results suggest that Tax-18 lacks a factor necessary for mitosis and that taxol may be able to substitute for this factor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.