ArticleThe Journal of cell biology1981
A permeabilized cell model for studying cell division: a comparison of anaphase chromosome movement and cleavage furrow constriction in lysed PtK1 cells.
Article in The Journal of cell biology, 1981. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 73 citations in OpenAlex.
- The perpetual movements of anaphase.Cellular and molecular life sciences : CMLS · 2010Review
- Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating spindle and kinetochore function.Molecular biology of the cell · 2006Article
- Organization of mammalian cytoplasm.Molecular and cellular biology · 2003Article
- The distribution, abundance and subcellular localization of kinesin.The Journal of cell biology · 1989Article
- Calmodulin stabilization of kinetochore microtubule structure to the effect of nocodazole.The Journal of cell biology · 1988Article
- Cleavage furrow isolated from newt eggs: contraction, organization of the actin filaments, and protein components of the furrow.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- The polarity and stability of microtubule capture by the kinetochore.The Journal of cell biology · 1988Article
- Physiological and ultrastructural analysis of elongating mitotic spindles reactivated in vitro.The Journal of cell biology · 1986Article
- Fluctuation of the Ca-sequestering activity of permeabilized sea urchin embryos during the cell cycle.Proceedings of the National Academy of Sciences of the United States of America · 1985Article
- Cytomatrix in chromatophores.The Journal of cell biology · 1984Review
- Taxol-requiring mutant of Chinese hamster ovary cells with impaired mitotic spindle assembly.The Journal of cell biology · 1983Article
- Cytoplasmic microtubule assembly-disassembly from endogenous tubulin in a Brij-lysed cell model.The Journal of cell biology · 1983Article
- Action of taxol on mitosis: modification of microtubule arrangements and function of the mitotic spindle in Haemanthus endosperm.The Journal of cell biology · 1983Article
- Taxol binds to cellular microtubules.The Journal of cell biology · 1982Article
- A functional in vitro model for studies of intracellular motility in digitonin-permeabilized erythrophores.The Journal of cell biology · 1982Article
- Evidence that myosin does not contribute to force production in chromosome movement.The Journal of cell biology · 1982Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
After lysis in a Brij 58-polyethylene glycol medium, PtK1 cells are permeable to small molecules, such as erythrosin B, and to proteins, such as rhodamine-labeled FAB, myosin subfragment-1, and tubulin. Holes are present in the plasma membrane, and the mitochondria are swollen and distorted, but other membrane-bounded organelles of the lysed cell model are not noticeably altered. After lysis, the mitotic apparatus is functional; chromosomes move poleward and the spindle elongates. Cells lysed while in cytokinesis will continue to divide for several minutes. Addition of crude tubulin extracts, MAP-free tubulin, or taxol to the lysis medium retards anaphase chromosome movements but does not affect cleavage. On the other hand, N-ethylmaleimide-modified myosin subfragment-1, phalloidin, and cytochalasin B inhibit cleavage but have no effect on anaphase chromosome movements under identical lysis conditions. These results suggest that actomyosin plays no functional role in anaphase chromosome movement in mammalian tissue culture cells and that microtubule depolymerization is a rate-limiting step for chromosome-to-pole movements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.