ArticleThe Journal of cell biology1984
Transient increase in intracellular pH during Dictyostelium differentiation.
Article in The Journal of cell biology, 1984. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 37 citations in OpenAlex.
- Ion Signaling in Cell Motility and Development inBiomolecules · 2024Review
- A developmentally regulated Na-H exchanger in Dictyostelium discoideum is necessary for cell polarity during chemotaxis.The Journal of cell biology · 2005Article
- The internal pH of the forespore compartment of Bacillus megaterium decreases by about 1 pH unit during sporulation.Journal of bacteriology · 1994Article
- Cytoplasmic pH of Dictyostelium discoideum amebae during early development: identification of two cell subpopulations before the aggregation stage.The Journal of cell biology · 1990Article
- Cytoplasmic pH regulation and chloride/bicarbonate exchange in avian osteoclasts.The Journal of clinical investigation · 1989Article
- Measurement of the cytoplasmic pH of Dictyostelium discoideum using a low light level microspectrofluorometer.The Journal of cell biology · 1988Article
- Effect of heavy chain phosphorylation on the polymerization and structure of Dictyostelium myosin filaments.The Journal of cell biology · 1987Article
- AMP deaminase in Dictyostelium discoideum: increase in activity following nutrient deprivation induced by starvation or hadacidin.Molecular and cellular biochemistry · 1986Article
- Intracellular pH in Dictyostelium discoideum: a 31P nuclear magnetic resonance study.The Journal of cell biology · 1985Article
- Preliminary evidence for a cholinergic-like system in lichen morphogenesis.The Histochemical journalArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The intracellular pH (pHi) of Dictyostelium discoideum amebae has been determined using the pH-dependent fluorescence of intracellularly trapped fluorescein (Thomas, J. A., R. N. Buschbaum, A. Zimiak, and E. Racker, Biochemistry, 18:2210-2218). The pHi of cells measured 45-60 min after initiation of differentiation was between 6.2 and 6.3. At approximately 2 h into differentiation cells underwent a transient intracellular alkalinization during which the pHi rose to 7.13 (+/- 0.3, n = 4), after which the pHi returned to approximately the original value (6.2-6.4). Cells that were removed from growth medium but were incubated in differentiation medium containing 3% dextrose did not exhibit this transient increase in pHi. The alkalinization event can also be prevented from occurring by differentiation in Na+-free solutions or by the addition of amiloride to sodium-containing buffer solutions, suggesting that the alkalinization is sodium dependent. When the alkalinization was prevented by amiloride treatment, cells did not progress normally into differentiation. This increase in pHi was initiated by the cells 2 h after removal from nutrient medium and it could be inhibited by several treatments that had been observed to delay the differentiation program, suggesting that it plays a major role in the initiation of the developmental program of this organism.
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