ArticleCell and tissue research1979
Effects of the actin-binding protein DNAase I on cytoplasmic streaming and ultrastructure of Amoeba proteus. An attempt to explain amoeboid movement.
Article in Cell and tissue research, 1979. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 38 citations in OpenAlex.
- Actin dynamics in Amoeba proteus motility.Protoplasma · 2007Article
- Amoeboid movement: a review and proposal of a 'membrane ratchet' model.Experientia · 1987Review
- Analysis of the role of microtubules and actin in erythrophore intracellular motility.The Journal of cell biology · 1983Article
- Dynamics of the cytoskeleton in Amoeba proteus. I. Redistribution of microinjected fluorescein-labeled actin during locomotion, immobilization and phagocytosis.Cell and tissue research · 1983Article
- Microinjection into an identified axon to study the mechanism of fast axonal transport.Proceedings of the National Academy of Sciences of the United States of America · 1982Article
- Effects of exogenous proteins on cytoplasmic streaming in perfused Chara cells.The Journal of cell biology · 1982Article
- Spatial organization and fine structure of the cortical filament layer in normal locomoting Amoeba proteus.Cell and tissue research · 1982Article
- Visualization of actin polymerization and depolymerization cycles during polyamine-induced cytokinesis in living Amoeba proteus.Cell and tissue research · 1981Article
- Analysis of the mechanism of fast axonal transport by intracellular injection of potentially inhibitory macromolecules: evidence for a possible role of actin filaments.Proceedings of the National Academy of Sciences of the United States of America · 1980Article
- Studies on microplasmodia of Physarum polycephalum. I. Classification and locomotion behavior.Cell and tissue research · 1980Article
- Organization and spatial arrangement of fluorescein-labeled native actin microinjected into normal locomoting and experimentally influenced Amoeba proteus.Cell and tissue research · 1980Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microinjection of DNAase I, which is known to form a specific complex with G-actin, induces characteristic changes in cytoplasmic streaming, locomotion and morphology of the contractile apparatus of A. proteus. Light microscopical studies show pronounced streaming originating from the uroid and/or the retracting pseudopods, which ceases 10--15 min after injection of DNAase I, at a time when ultrasctructural studies show that the actin filament system is very much reduced. These results suggest that a controlled reversible equilibrium between soluble and polymerized forms of actin is a necessary requirement for amoeboid movement. The topographic distribution of contractile filaments beneath the plasma membrane visualized by correlated light- and electron microscopy of DNAase I-injected cells establishes the importance of the membrane-bound filamentous layer for three major aspects of streaming: (1) Streaming originates by local contractions of a cell membrane-associated filament layer at the uroid and/or retracting pseudopods, creating a pressure flow. (2) This flow continues beneath the membrane, which is stabilized by filaments in the lateral regions between the posterior end, with a high hydrostatic pressure, and the anterior end, with a low hydrostatic pressure. (3) Pseudopods or extending areas are created by a local destabilization of the cell periphery caused by the separation of the filamentous layer from the plasma membrane.
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