ArticleThe Journal of cell biology1978
Actin in Xenopus oocytes. II. Intracellular distribution and polymerizability.
Article in The Journal of cell biology, 1978. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 54 citations in OpenAlex.
- Nuclear actin filaments - a historical perspective.Nucleus (Austin, Tex.) · 2024Review
- Controlled aggregation of ferritin to modulate MRI relaxivity.Biophysical journal · 2008Article
- Mutant Caldesmon lacking cdc2 phosphorylation sites delays M-phase entry and inhibits cytokinesis.Molecular biology of the cell · 2001Article
- Mechanically gated channel activity in cytoskeleton-deficient plasma membrane blebs and vesicles from Xenopus oocytes.The Journal of physiology · 2000Article
- Actin and actin-associated proteins in Xenopus eggs and early embryos: contribution to cytoarchitecture and gastrulation.Current topics in developmental biology · 1992Review
- Proteins regulating actin assembly in oogenesis and early embryogenesis of Xenopus laevis: gelsolin is the major cytoplasmic actin-binding protein.The Journal of cell biology · 1988Article
- Diffusive and nondiffusive proteins in vivo.The Journal of cell biology · 1984Article
- Monomer-polymer equilibria in the axon: direct measurement of tubulin and actin as polymer and monomer in axoplasm.The Journal of cell biology · 1984Article
- An actin-depolymerizing protein (depactin) from starfish oocytes: properties and interaction with actin.The Journal of cell biology · 1983Article
- A nucleolar skeleton of protein filaments demonstrated in amplified nucleoli of Xenopus laevis.The Journal of cell biology · 1981Article
- Mechanism for the selection of nuclear polypeptides in Xenopus oocytes. II. Two-dimensional gel analysis.The Journal of cell biology · 1980Article
- Effect of microinjected N-ethylmaleimide-modified heavy meromyosin on cell division in amphibian eggs.The Journal of cell biology · 1980Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The largest oocytes of Xenopus Laevis were broken open in the absence of shearing forces which might transfer actin from particulate to supernatant fractions. Particulate and postmitochondrial supernatant fractions were prepared by centrifugation. SDS-electrophoretic fractionation on polyacrylamide gels and quantitative scanning techniques were used to separate actin and to assay its amount in cellular fractions. The actin has been identified in electrophoretograms by its molecular weight and its binding to DNase I. oocytes contain 1.4-1.7 {um}g of actin per cell, of which up to 88 percent is recovered in the postmitochondrial supernate under a variety of conditions. In the soluble fraction, it represents about 8.8 percent of the total protein. Its concentration in native cytoplasm was directly assayed at 4.1 mg/ml. There is no detectable actin that can be transferred from the particulate to the soluble phase by neutral detergents or ionic conditions that would depolymerize muscle actin. Centrifugation of the soluble oocyte fractions showed that 75-95 percent of the actin can not be sedimented under forces that would pellet filamentous actin. Addition of potassium and magnesium to the cytoplasm, to concentrations that would polymerize muscle actin, does not increase the amount of sedimentable actin. Roughly one-third of the soluble actin is recovered from Sephadex columns at about the position of monomer. About two- thirds is in complexes of 100,000 daltons or greater.
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