ArticleThe Journal of cell biology1978
Actin in Xenopus oocytes.
Article in The Journal of cell biology, 1978. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 57 citations in OpenAlex.
- Nuclear actin filaments - a historical perspective.Nucleus (Austin, Tex.) · 2024Review
- Review
- Tug-of-war between actomyosin-driven antagonistic forces determines the positioning symmetry in cell-sized confinement.Nature communications · 2020Article
- Analysis of nuclear actin by overexpression of wild-type and actin mutant proteins.Histochemistry and cell biology · 2014Article
- Actin behavior in bulk cytoplasm is cell cycle regulated in early vertebrate embryos.Journal of cell science · 2011Article
- Differential sublocalization of actin variants within the nucleus.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Mechanical properties of Xenopus egg cytoplasmic extracts.Biophysical journal · 2005Article
- Actin and actin-associated proteins in Xenopus eggs and early embryos: contribution to cytoarchitecture and gastrulation.Current topics in developmental biology · 1992Review
- Proteins regulating actin assembly in oogenesis and early embryogenesis of Xenopus laevis: gelsolin is the major cytoplasmic actin-binding protein.The Journal of cell biology · 1988Article
- Diffusive and nondiffusive proteins in vivo.The Journal of cell biology · 1984Article
- Characterization and localization of actinogelin, a Ca2+ - sensitive actin accessory protein, in nonmuscle cells.The Journal of cell biology · 1982Article
- Induction of either contractile or structural actin-based gels in sea urchin egg cytoplasmic extract.The Journal of cell biology · 1980Article
- Isolation of a high molecular weight actin-binding protein from baby hamster kidney (BHK-21) cells.Proceedings of the National Academy of Sciences of the United States of America · 1979Article
- Actin in Xenopus oocytes. II. Intracellular distribution and polymerizability.The Journal of cell biology · 1978Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been found that a high-speed supernatant fraction from Xenopus oocytes extracted in the cold will form a clear, solid gel upon warming. Gel formation occurs within 60 min at 18 degrees-40 degrees C, and is, at least initially, temperature reversible. Gelation is strictly dependent upon the addition of sucrose to the extraction medium. When isolated in the presence of ATP, the gel consists principally of a 43,000-dalton protein which co-migrates with Xenopus skeletal muscle actin on SDS-polyacrylamide gels, and a prominent high molecular weight component of approx. 250,000 daltons. At least two minor components of intermediate molecular weight are also found associated with the gel in variable quantities. Actin has been identified as the major consituent of the gel by ultrastructural and immunological techniques, and comprises roughly 47% of protein in the complex. With time, the gel spontaneously contracts to form a small dense aggregate. Contraction requires ATP. In the absence of exogenous ATP, a polypeptide which co-migrates with the heavy chain of Xenopus skeletal muscle myosin becomes a prominent component of the gel. This polypeptide is virtually absent from gels which have contracted in ATP-containing extracts. It has also been found that Ca++ is required for gelation in oocyte extracts. At both low and high concentrations of Ca++ (defined as a ratio of Ca++/EGTA in the extraction medium), gelation is inhibited.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.