Evidence map›Paper›PMID 42919911›Full record

ArticleNature neuroscience2026

Identification of candidate markers for the isolation of neuron-specific extracellular vesicles.

Dmitry Ter-Ovanesyan, Sara Whiteman, Tal Gilboa, Emma J K Kowal, Wendy Trieu, Siddharth Iyer, Bogdan Budnik, Clarissa May Babila, Chungmin Han, David Vance and 7 more

Abstract read
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In one paragraph

Article in Nature neuroscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Dmitry Ter-OvanesyanWyss Institute for Biologically Inspired Engineering, Boston, MA, USA. dter@wyss.harvard.edu.ORCID http://orcid.org/0000-0002-1134-0073
Sara WhitemanWyss Institute for Biologically Inspired Engineering, Boston, MA, USA.
Tal GilboaWyss Institute for Biologically Inspired Engineering, Boston, MA, USA.
Emma J K KowalWyss Institute for Biologically Inspired Engineering, Boston, MA, USA.
Wendy TrieuWyss Institute for Biologically Inspired Engineering, Boston, MA, USA.
Siddharth IyerWyss Institute for Biologically Inspired Engineering, Boston, MA, USA.
Bogdan BudnikWyss Institute for Biologically Inspired Engineering, Boston, MA, USA.ORCID http://orcid.org/0000-0003-3622-2003
Clarissa May BabilaWyss Institute for Biologically Inspired Engineering, Boston, MA, USA.
Chungmin HanWyss Institute for Biologically Inspired Engineering, Boston, MA, USA.ORCID http://orcid.org/0000-0001-8266-318X
David VanceWyss Institute for Biologically Inspired Engineering, Boston, MA, USA.
Graham HeimbergGenentech, South San Francisco, CA, USA.
Michael W BurgessBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Hasmik KeshishianBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Steven A CarrBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-7203-4299
Aviv Regev *Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. aviv.regev.sc@gmail.com.ORCID http://orcid.org/0000-0003-3293-3158
George M Church *Wyss Institute for Biologically Inspired Engineering, Boston, MA, USA. gchurch@genetics.harvard.med.edu.ORCID http://orcid.org/0000-0001-6232-9969
David R Walt *Wyss Institute for Biologically Inspired Engineering, Boston, MA, USA. dwalt@bwh.harvard.edu.ORCID http://orcid.org/0000-0002-5524-7348

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular vesicles (EVs) are present in biofluids and may be useful biomarkers to non-invasively monitor brain cells, but isolating cell type-specific EVs remains challenging. Here we introduce an approach to immuno-isolate EVs from neurons and glia based on protein surface markers. We first selected transmembrane proteins suitable as cell type-specific EV markers based on gene expression and EV proteomic data. We developed a high-purity EV isolation method and cataloged the proteins present on EVs in human cerebrospinal fluid and plasma. We then computationally identified cell type-specific candidate markers for isolating EVs from neurons, astrocytes, oligodendrocytes and microglia. Using ultrasensitive immunoassays against several predicted neuron-specific proteins, we confirmed one marker, NRXN3, as present on EVs in cerebrospinal fluid and plasma. Finally, we developed efficient EV immuno-isolation methods and applied them to isolate NRXN3

Identifiers

PMID42919911

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.