Application of Restriction Fragment Length Polymorphism (RFLP).

Muhammad Mumtaz Khan, Rashad Waseem Qadri, Muhammad Tahir Akram, Abdul Haseeb Ahmed, Abdullah Al-Sadi

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Muhammad Mumtaz KhanDepartment of Plant Sciences, College of Agricultural and Marine Science, Sultan Qaboos University, Al-Khoud 123, P.O. Box 34, Muscat, Oman. mumtaz@squ.edu.om.

Rashad Waseem QadriInstitute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan.

Muhammad Tahir AkramDepartment of Horticulture, PMAS-Arid Agriculture University, Rawalpindi, Pakistan.

Abdul Haseeb AhmedInstitute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan.

Abdullah Al-SadiDepartment of Plant Sciences, College of Agricultural and Marine Science, Sultan Qaboos University, Al-Khoud 123, P.O. Box 34, Muscat, Oman.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Restriction fragment length polymorphism (RFLP) was the first reliable genetic marker used in plant molecular studies. It is a DNA-based marker that detects variation in DNA sequence at restriction enzyme sites and produces codominant, locus-specific markers that differentiate homologous and heterozygous genotypes. It provides greater accuracy than trait-based methods. The application of RFLP markers includes developing genetic linkage maps, conducting QTL analysis, characterizing germplasm, and detecting somaclonal variations. Despite being the availability of new molecular markers, RFLP still remained highly valued for its reproducibility and clear Mendelian inheritance patterns. This chapter presents optimized, nonradioactive protocols for DNA isolation, Southern blotting, DIG-labeled probe hybridization, and chemiluminescent detection, offering a practical guideline for integrating RFLP markers into modern plant breeding and genomic research.

Indexed as

PlantsPolymorphism, Restriction Fragment LengthBlotting, SouthernChromosome MappingDNA, PlantGenetic MarkersQuantitative Trait LociDNA, PlantGenetic MarkersCo-dominant inheritanceGenetic mappingMolecular markersQTL analysisSouthern blotting

Identifiers

PMID42919028

DOI10.1038/s41598-025-15598-1

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.