Evidence map›Paper›PMID 42919020›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Development and Application of Expressed Sequence Tags (ESTs) for the Improvement of Cotton Crops.

Gokul Murugesan, Tanu Priya Natarajan, Janani Sree Sivakumar, Dhandapani Gurusamy, Kanakachari Mogilicherla, Vasanth Krishnan

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Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Gokul MurugesanDepartment of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India.
Tanu Priya NatarajanDepartment of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India.
Janani Sree SivakumarDepartment of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India.
Dhandapani GurusamyDepartment of Botany, Kongunadu Arts and Science College (Autonomous), Bharathiar University, Coimbatore, Tamil Nadu, India. gdhandapani@kongunaducollege.ac.in.
Kanakachari MogilicherlaDepartment of Biotechnology, ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, Telangana, India.
Vasanth KrishnanDepartment of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India. krishnanvasanth1975@buc.edu.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Expressed sequence tags (ESTs), are short cDNA sequences obtained from expressed genes in any plant tissue of Gossypium spp. and represent a suitable approach for studying the transcriptome in the absence of a reference genome. In this study, protocols for EST development were established to explore expressed genes in cotton under diverse biotic and abiotic stress conditions. ESTs were generated by using two approaches: suppression subtractive hybridization (SSH) and next-generation sequencing (NGS) techniques. The process begins with RNA isolation, followed by mRNA purification, cDNA synthesis, and cloning, leading to the generation of single-pass EST libraries. High-quality ESTs were assembled into unigenes using complementary assembly strategies. Functional annotation of the resulting unigenes was carried out to enhance gene representation and to elucidate stress-responsive pathways. This approach supports key objectives in cotton breeding, including improvement of fiber quality and productivity, as well as enhanced resistance to biotic and abiotic stresses.

Indexed as

Crops, AgriculturalExpressed Sequence TagsGossypiumCloning, MolecularDNA, ComplementaryGene Expression ProfilingGene Expression Regulation, PlantGene LibraryHigh-Throughput Nucleotide SequencingStress, PhysiologicalSubtractive Hybridization TechniquesDNA, ComplementaryCloningComplementary DNACottonExpressed sequence tagsMacroarrayNext generation sequencingSuppression subtractive hybridizationTotal RNA

Identifiers

PMID42919020

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.