ArticleMethods in molecular biology (Clifton, N.J.)2027
RAPD Markers for the Study of Genetic Diversity in Echium amoenum Ecotypes.
Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This chapter details a comprehensive, step-by-step RAPD protocol for assessing genetic diversity in 16 Iranian Echium amoenum ecotypes, a medicinal plant. The method uses STE (Sucrose-Tris-EDTA) buffer to extract DNA from leaf tissue, followed by spectrophotometric and electrophoretic quality control. PCR amplification with 20 decamer primers yields 248 polymorphic bands (80% polymorphism), separated by agarose gel electrophoresis. NTSYS-pc analysis, using the Dice coefficient, UPGMA clustering, and principal component analysis, reveals geographic-climatic groupings. This approach is applicable not only to E. amoenum but also to other less-studied medicinal plants.
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