ArticleMethods in molecular biology (Clifton, N.J.)2027
Experimental Model to Study Microbiota Perturbation Role in Alpha-Gal Syndrome.
Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This chapter describes a standardized experimental protocol to induce and analyze galactose-α-1,3-galactose (α-Gal)-specific IgE responses in α-Gal-deficient (α1,3-galactosyltransferase knockout) mice. The absence of endogenous α-Gal in this model enables controlled sensitization to exogenous α-Gal-containing antigens from microbial, arthropod, and mammalian sources. The protocol integrates sequential oral exposure to α-Gal-expressing Escherichia coli O86:B7, capsule-based infestation with Ixodes ricinus nymphs, oral administration of porcine tissue homogenate, and subcutaneous injection of tick-derived protein extracts. Step-by-step procedures are provided for antigen preparation, animal handling, tick infestation, blood sampling, and indirect ELISA-based quantification of α-Gal-specific IgE. The protocol is designed for repeated sampling over time following defined antigen exposures and can be applied as an in vivo experimental framework for studies of α-Gal-associated IgE responses.
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