ArticleBrazilian oral research2026
Effect of LPS and IFN-γ transient inflammatory priming on periodontal ligament cells and Schwann cells.
Article in Brazilian oral research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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11 authors.
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Abstract
This study investigated the effects of Escherichia coli lipopolysaccharide (LPS; 2,000 ng/mL) and interferon-γ (IFN-γ; 50 ng/mL) on human periodontal ligament cells (PDLCs) and mouse Schwann cells (SCs). After a transient inflammatory priming (5 hours), cell migration/proliferation and viability (n = 4) were assessed with scratch and MTT assays, respectively, while inflammatory markers were quantified by real-time quantitative polymerase chain reaction (RT-qPCR; n = 3). Mineralized matrix formation was induced for 12 days and analyzed via Alizarin Red staining (ARS; n = 4) and osteogenesis-related gene expression (RT-qPCR; n = 3). Because of their distinct origins, the two cell lineages were assessed independently. Exposure to LPS+IFN-γ did not affect the viability or migration/proliferation of PDLCs or SCs. In PDLCs, LPS+IFN-γ exposure upregulated interleukin-1α (IL-1α) and interleukin-6 (IL-6), interferon-γ (IFN-γ), IFN-γ receptor (IFN-γR), and tumor necrosis factor (TNF). In SCs, IL-6 and IFN-γR were upregulated, whereas TNF was downregulated compared to the untreated control. Following mineral matrix induction, exposed PDLCs showed increased expression of alkaline phosphatase (ALPL) and runt-related transcription factor 2 (RUNX-2). SCs upregulated ALPL, RUNX-2, and osteocalcin (OCN). Quantitative ARS data indicated that calcium deposition in PDLCs exposed to LPS+IFN-γ was similar to that in the untreated group. SCs exposed to LPS+IFN-γ exhibited significant mineralized matrix formation. Overall, PDLCs responded to LPS+IFN-γ priming with an inflammatory transcriptional response. SCs were unresponsive or showed a downregulated response to inflammatory markers (TNF and IL-1α). Both cell lineages demonstrated mineralized matrix formation after LPS and IFN-γ transient priming.
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