ArticleMolecular biology reports2026
Modulation of the NAMPT/SIRT axis in HeLa cells exposed to adipose-derived mesenchymal stem cell-conditioned medium.
Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundCervical cancer cell survival depends on coordinated metabolic and stress response pathways. Adipose tissue-derived mesenchymal stem/stormal cells (AD-MSCs) release soluble factors and extracellular vesicle cargo that can alter tumor cell behavior, but their effects on the NAMPT/sirtuin axis in cervical cancer cells are not well defined. We therefore examined how AD-MSC-conditioned medium (CM) affects HeLa cell viability, apoptosis, and expression of NAMPT, SIRT1, SIRT2, and SIRT7.
methodsPreviously isolated, characterized, and cryopreserved human AD-MSCs were thawed, expanded, and used to prepare CM. HeLa cells were exposed to 10%-100% CM for 48 h, and viability was measured by MTT assay. The 20% and 60% CM conditions were then evaluated by Annexin V/PI flow cytometry and qRT-PCR. NAMPT, SIRT1, SIRT2, and SIRT7 transcripts were quantified relative to GAPDH.
resultsCM exposure reduced HeLa cell viability, with an estimated IC50 at 38.99%. The 20% condition increased the apoptotic cell fraction, whereas the response to 60% CM was not significant. At 20% CM, SIRT2 (p = 0.0036) expression decreased, SIRT7 expression increased (p = 0.0036), SIRT1 (p = 0.6286) and NAMPT(p = 0.3277) expression did not differ significantly from the comparison groups.
conclusionUnder the conditions tested, AD-MSC-CM reduced HeLa cell viability and promoted apoptosis most clearly at 20% CM. The accompanying decrease in SIRT2 and increase in SIRT7 point to a paracrine response involving metabolic and sirtuin related signaling. Identification of the active CM components and confirmation at the protein, enzyme-activity, and pathway levels are required before a causal mechanism can be assigned.
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