ArticleProbiotics and antimicrobial proteins2026
Integrated Phenotypic and Genomic Characterization Reveals Functional Heterogeneity among Four Bifidobacterium Strains.
Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the genus Bifidobacterium exhibit substantial inter- and intraspecific variation in probiotic-associated functions, emphasizing the need for integrated phenotypic and genomic evaluation. This study comparatively characterized four Bifidobacterium strains from the Thailand Institute of Scientific and Technological Research (TISTR) Culture Collection using phenotypic and genomic approaches. Hybrid genome assembly generated complete circular chromosomes, and genome-based safety assessment detected no recognized virulence-associated genes. All strains were predicted to be non-human pathogens and were phenotypically susceptible to the eight antimicrobials tested. Although tet(W)-associated sequences were identified in TISTR 2591 and TISTR 2893, neither strain exhibited phenotypic tetracycline resistance. Comparative CAZyme analysis identified 71-82 high-confidence CAZyme-encoding genes, with TISTR 2893 possessing the largest and most diverse glycoside hydrolase repertoire. Distinct functional profiles were observed among the four strains. TISTR 2591 exhibited the strongest epithelial adhesion, reaching 19.05% and 10.47% to Caco-2 and HT-29 cells, respectively, together with broad carbohydrate utilization and predominantly L-lactate production. TISTR 2737 showed the greatest gastric-acid tolerance among the TISTR strains, with a 0.34-log₁₀ CFU/mL reduction after 180 min at pH 2.0, whereas TISTR 2986 showed the greatest bile-salt tolerance, with a 0.50-log₁₀ CFU/mL reduction. TISTR 2893 exhibited the broadest antagonistic spectrum, inhibiting all six indicator bacteria. Neutralization of the cell-free supernatants abolished detectable inhibition, while genome mining revealed no convincing complete bacteriocin biosynthetic cluster. Under the trait-weighted functional screening framework, TISTR 2893 obtained the highest numerical score (15/28; 53.57%), whereas the equal-domain sensitivity analysis produced a different numerical ordering, demonstrating the influence of weighting structure on composite evaluation. Collectively, the four strains exhibited distinct functional profiles rather than a uniform hierarchy of probiotic-associated performance, supporting function-specific strain selection based on complementary phenotypic and genomic evidence.
Indexed as
Identifiers
42909839What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.