ArticleMarine biotechnology (New York, N.Y.)2026
Genome-wide DNA Methylation Difference Between Sexes in Juveniles and Epigenetic Regulation of Sexual Development in Intersex Crabs Eriocheir sinensis.
Article in Marine biotechnology (New York, N.Y.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Epigenetic regulation of sexual development is widely documented in model species, yet data for commercial invertebrates remain limited. Here, we used whole-genome bisulfite sequencing (WGBS) to profile genome-wide DNA methylation and identify differentially methylated regions (DMRs) between the female and male juveniles at stage III in the Chinese mitten crab Eriocheir sinensis. Global methylated cytosines accounted for approximately 1.44% and 1.45% of all cytosines in the female and male genomes, respectively. Among the CpG, CHG, and CHH contexts, CpG showed the highest mean methylation levels, at 8.15% in females and 8.08% in males. A total of 21,038 nominally significant DMRs were identified between sexes, with 11,557 (55%) hypermethylated DMRs in males. Notably, the methylation level of the ZW chromosome was the highest compared to the ZZ chromosome and the autosomes, suggesting sex-biased epigenetic modification within the ZW/ZZ sex determination system. On autosomes, the hypermethylated DMRs in females were mainly detected in the gene body regions of Sex lethal, Male-specific lethal 3, Feminization-1c, and Feminization-1a. On the ZW chromosome, 233 DMRs were hypermethylated in females, whereas 161 were hypermethylated in males. Furthermore, in the intersex crabs (ZW) bearing male first gonopods, the promoter of LOC113817324 exhibited intersex-specific hypermethylation in the ovary, whereas intronic methylation of the Serine/threonine-protein kinase (ATR) remained low, mirroring the pattern observed in normal male (ZZ) testes. These findings reveal sex- and intersex-associated DNA methylation patterns in E. sinensis and provide candidate epigenetic loci for further investigation of sexual development.
Indexed as
Identifiers
42909566What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.