Evidence map›Paper›PMID 42909045›Full record

ArticleFrontiers in immunology2026

Chloroquine inhibits Spring viremia of carp virus replication by modulating autophagy and metabolic pathways.

Yuchao Zhu, Shuyang Chen, Yue Wang, Jian Shen, Libo He, Yaping Wang, Dong Wang, Shuyan Miao, Guobin Chang, Pengfei Chu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yuchao Zhu *College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Shuyang Chen *College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Yue WangCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Jian ShenCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Libo HeState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Yaping WangState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Dong WangYizheng Water World Agricultural Ecological Park Co., Ltd, Yangzhou, China.
Shuyan MiaoCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Guobin ChangCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Pengfei ChuCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Spring viremia of carp virus (SVCV) severely threatens cyprinid aquaculture, yet the role of autophagy in SVCV infection remains unclear. Here, SVCV infection was associated with enhanced autophagic activity and autophagic flux in common carp and EPC cells. Pharmacological perturbation of autophagy revealed that rapamycin treatment increased SVCV gene expression and aggravated SVCV-induced cytopathic effects, whereas chloroquine blocked autophagic flux, suppressed viral gene expression, and mitigated virus-induced cellular damage. Transcriptomic analysis identified 1,868 differentially expressed genes after chloroquine treatment, with major changes in lysosome/autophagy-related pathways, glycan degradation, ion transport, cell cycle, DNA replication, and DNA repair. Metabolomic and integrated multi-omics analyses revealed changes in amino sugar and nucleotide sugar metabolism, steroid biosynthesis, riboflavin metabolism, and amino acid metabolism. Functional validation showed that diflorasone and all-trans-retinal reduced SVCV-G expression, alleviated cytopathic effects, and improved survival after SVCV infection. These findings suggest that SVCV infection is associated with enhanced autophagic flux, whereas chloroquine treatment blocks autophagic flux and is accompanied by reduced SVCV gene expression.

Indexed as

Antiviral AgentsAutophagyCarpsChloroquineFish DiseasesMetabolic Networks and PathwaysRhabdoviridaeRhabdoviridae InfectionsVirus ReplicationAnimalsCell LineAntiviral AgentsChloroquineautophagychloroquinemetabolomicsSVCVtranscriptomics

Identifiers

PMID42909045
PMCPMC13683750

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.