Evidence map›Paper›PMID 42908790›Full record

ArticleFood chemistry: X2026

Adsorption-crosslinking immobilization of SHSL on NKA macroporous resin: Operational stability and molecular dynamics at the enzyme-resin interface.

Wei Wu, Yunzhu Chen, Peiwang Li, Jiali Ren, Wenyan Yuan, Yan Yang, Zhihong Xiao, Xiao Zhou, Yan Chao, Shengyu Guo and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Food chemistry: X, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Wei WuState Key Laboratory of Woody Oil Resources Utilization, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, 498 South Shaoshan Road, Changsha, Hunan 410004, PR China.
Yunzhu ChenHunan Academy of Forestry, Changsha, Hunan 410004, PR China.
Peiwang LiHunan Academy of Forestry, Changsha, Hunan 410004, PR China.
Jiali RenState Key Laboratory of Woody Oil Resources Utilization, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, 498 South Shaoshan Road, Changsha, Hunan 410004, PR China.
Wenyan YuanState Key Laboratory of Woody Oil Resources Utilization, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, 498 South Shaoshan Road, Changsha, Hunan 410004, PR China.
Yan YangHunan Academy of Forestry, Changsha, Hunan 410004, PR China.
Zhihong XiaoHunan Academy of Forestry, Changsha, Hunan 410004, PR China.
Xiao ZhouHunan Academy of Forestry, Changsha, Hunan 410004, PR China.
Yan ChaoHunan Academy of Forestry, Changsha, Hunan 410004, PR China.
Shengyu GuoDepartment of economics and management, ChangSha University, Changsha, Hunan 410022, PR China.
Jingzhen ChenHunan Academy of Forestry, Changsha, Hunan 410004, PR China.
Changzhu LiState Key Laboratory of Woody Oil Resources Utilization, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, 498 South Shaoshan Road, Changsha, Hunan 410004, PR China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lipase immobilization can improve operational stability, while enzyme-support interactions may alter conformational behavior near the catalytic region. How local support association relates to catalytic-region dynamics remains unclear. Here, SHSL was immobilized on NKA macroporous resin by hydrophobic adsorption followed by glutaraldehyde post-treatment. The optimized preparation showed 91.44 ± 2.39 U/g activity, 65.47% immobilization yield, and 26.2% activity recovery, retaining 53.7% activity after 10 reuse cycles and 61.0% after 35 days of storage versus 11.7% for free SHSL. Glutaraldehyde post-treatment reduced cumulative protein leakage after 10 cycles from 40.14% to 15.38% relative to adsorption alone. In 150-ns molecular dynamics simulations, a representative SHSL-NKA fragment complex maintained persistent noncovalent association away from the catalytic triad. During 50-150 ns, interfacial fluctuations decreased by 31.7%, whereas lid mobility increased by 17.6%. NKA-fragment association also increased lid opening, decreased pocket volume, altered catalytic-residue geometry, and redistributed access-channel occurrence.

Indexed as

Access channelsInterfacial dynamicsLipase immobilizationMacroporous resinMolecular dynamics

Identifiers

PMID42908790
PMCPMC13683448

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